Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18562 | 5' | -54.6 | NC_004682.1 | + | 10549 | 0.69 | 0.584475 |
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Target: 5'- uUCGGUCAcacCGUCGAAGaGAU-GACCa- -3' miRNA: 3'- cAGCCAGU---GCAGCUUC-CUGuCUGGcg -5' |
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| 18562 | 5' | -54.6 | NC_004682.1 | + | 22552 | 0.7 | 0.530652 |
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Target: 5'- cGUUGGg--UGUCGAAGGACAcGCCGg -3' miRNA: 3'- -CAGCCaguGCAGCUUCCUGUcUGGCg -5' |
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| 18562 | 5' | -54.6 | NC_004682.1 | + | 24041 | 0.71 | 0.478738 |
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Target: 5'- cUCGcacCGCGUgcugCGggGGAUGGACCGCu -3' miRNA: 3'- cAGCca-GUGCA----GCuuCCUGUCUGGCG- -5' |
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| 18562 | 5' | -54.6 | NC_004682.1 | + | 10038 | 0.71 | 0.468651 |
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Target: 5'- -aCGGUgGCGcUCGggGGgcugacgacaucGCAGACCGa -3' miRNA: 3'- caGCCAgUGC-AGCuuCC------------UGUCUGGCg -5' |
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| 18562 | 5' | -54.6 | NC_004682.1 | + | 11163 | 0.75 | 0.27253 |
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Target: 5'- -cCGGgagUACGUCGAGGGACu--CCGCu -3' miRNA: 3'- caGCCa--GUGCAGCUUCCUGucuGGCG- -5' |
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| 18562 | 5' | -54.6 | NC_004682.1 | + | 32678 | 0.75 | 0.265688 |
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Target: 5'- aGUUGGUCAUGuUCGGcAGGACcgcccuGGCCGCu -3' miRNA: 3'- -CAGCCAGUGC-AGCU-UCCUGu-----CUGGCG- -5' |
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| 18562 | 5' | -54.6 | NC_004682.1 | + | 38433 | 1.1 | 0.000999 |
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Target: 5'- gGUCGGUCACGUCGAAGGACAGACCGCc -3' miRNA: 3'- -CAGCCAGUGCAGCUUCCUGUCUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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