Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 18564 | 3' | -52.6 | NC_004682.1 | + | 44065 | 0.72 | 0.504562 |
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Target: 5'- cUUGUCGCCGUCCGUGAUGgaggcguACAugucACCAGc -3' miRNA: 3'- -AGCAGUGGCAGGUGCUAC-------UGU----UGGUC- -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 38371 | 0.73 | 0.468195 |
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Target: 5'- cUCGUCcCCGUCCGCGAgGAUGAagacuucccgguaccCCAGg -3' miRNA: 3'- -AGCAGuGGCAGGUGCUaCUGUU---------------GGUC- -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 44405 | 0.73 | 0.444052 |
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Target: 5'- cUGgCACCGUCCAUGAUcGAgGACCAc -3' miRNA: 3'- aGCaGUGGCAGGUGCUA-CUgUUGGUc -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 10025 | 0.75 | 0.343167 |
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Target: 5'- aUCGUgGUCGUCCACGGUGGCGcUCGGg -3' miRNA: 3'- -AGCAgUGGCAGGUGCUACUGUuGGUC- -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 37406 | 0.79 | 0.210231 |
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Target: 5'- gUGUUGCCGUCCucguCGGUGACGACCu- -3' miRNA: 3'- aGCAGUGGCAGGu---GCUACUGUUGGuc -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 35890 | 1.1 | 0.00157 |
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Target: 5'- cUCGUCACCGUCCACGAUGACAACCAGg -3' miRNA: 3'- -AGCAGUGGCAGGUGCUACUGUUGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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