Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 18564 | 3' | -52.6 | NC_004682.1 | + | 29265 | 0.66 | 0.853317 |
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Target: 5'- aUCGacUCACCGcccgCCACGAagGACGuAUCAGu -3' miRNA: 3'- -AGC--AGUGGCa---GGUGCUa-CUGU-UGGUC- -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 20195 | 0.66 | 0.853317 |
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Target: 5'- gCGUC-CUGgCCGCGcugucgGGCGACCGGa -3' miRNA: 3'- aGCAGuGGCaGGUGCua----CUGUUGGUC- -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 22135 | 0.66 | 0.835746 |
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Target: 5'- uUCGUCccGCCGccgCCGCGAgugGGCGGCa-- -3' miRNA: 3'- -AGCAG--UGGCa--GGUGCUa--CUGUUGguc -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 46186 | 0.66 | 0.826625 |
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Target: 5'- gUCGUCAccCCGUCCGgGGUucucGAUGGCCu- -3' miRNA: 3'- -AGCAGU--GGCAGGUgCUA----CUGUUGGuc -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 6960 | 0.66 | 0.826625 |
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Target: 5'- ---aCACCGUCCugGAgGAguACCGc -3' miRNA: 3'- agcaGUGGCAGGugCUaCUguUGGUc -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 50169 | 0.66 | 0.865053 |
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Target: 5'- gUCGUCACCacgacggauGUggucgaccucggucgCCugGGUGagGCAACCGGa -3' miRNA: 3'- -AGCAGUGG---------CA---------------GGugCUAC--UGUUGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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