Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18564 | 3' | -52.6 | NC_004682.1 | + | 10995 | 0.7 | 0.648502 |
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Target: 5'- aUCGUC-CCGUUCGCGGcUGGCAagaGCgCAGc -3' miRNA: 3'- -AGCAGuGGCAGGUGCU-ACUGU---UG-GUC- -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 10025 | 0.75 | 0.343167 |
|
Target: 5'- aUCGUgGUCGUCCACGGUGGCGcUCGGg -3' miRNA: 3'- -AGCAgUGGCAGGUGCUACUGUuGGUC- -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 9296 | 0.7 | 0.626159 |
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Target: 5'- -gGUCAuCUGgaaCCGCGAgGACGGCCAGu -3' miRNA: 3'- agCAGU-GGCa--GGUGCUaCUGUUGGUC- -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 8310 | 0.71 | 0.592709 |
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Target: 5'- aCGcCACCGUCCGCucGAUGuucGCCAGc -3' miRNA: 3'- aGCaGUGGCAGGUG--CUACuguUGGUC- -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 6960 | 0.66 | 0.826625 |
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Target: 5'- ---aCACCGUCCugGAgGAguACCGc -3' miRNA: 3'- agcaGUGGCAGGugCUaCUguUGGUc -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 3036 | 0.67 | 0.797056 |
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Target: 5'- cCGUCACCGUUCgaucaggACGA-GGCGGCUg- -3' miRNA: 3'- aGCAGUGGCAGG-------UGCUaCUGUUGGuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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