Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18564 | 3' | -52.6 | NC_004682.1 | + | 42760 | 0.66 | 0.835746 |
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Target: 5'- -gGUCAggaCGUgCCACGcgGGCAgACCGGg -3' miRNA: 3'- agCAGUg--GCA-GGUGCuaCUGU-UGGUC- -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 43545 | 0.66 | 0.825701 |
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Target: 5'- cCGUCGCUGUCCcucagcACGGUGuCGAuguagccCCGGa -3' miRNA: 3'- aGCAGUGGCAGG------UGCUACuGUU-------GGUC- -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 21901 | 0.67 | 0.788137 |
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Target: 5'- gCGUucaCGCUGUCaGCGuuccUGGCGACCAGg -3' miRNA: 3'- aGCA---GUGGCAGgUGCu---ACUGUUGGUC- -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 49961 | 0.67 | 0.778069 |
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Target: 5'- cUGUgGCCGUCC-CGAUGAgCAugCu- -3' miRNA: 3'- aGCAgUGGCAGGuGCUACU-GUugGuc -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 26244 | 0.68 | 0.767845 |
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Target: 5'- cUCGgUGCCGUCCGCGA--GCuuCCGGu -3' miRNA: 3'- -AGCaGUGGCAGGUGCUacUGuuGGUC- -5' |
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| 18564 | 3' | -52.6 | NC_004682.1 | + | 38371 | 0.73 | 0.468195 |
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Target: 5'- cUCGUCcCCGUCCGCGAgGAUGAagacuucccgguaccCCAGg -3' miRNA: 3'- -AGCAGuGGCAGGUGCUaCUGUU---------------GGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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