Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18566 | 3' | -53.4 | NC_004682.1 | + | 38286 | 0.71 | 0.526959 |
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Target: 5'- aGUUCACGUCuucgccUGGUGGCAUCGgGAUg -3' miRNA: 3'- -CAAGUGUAGcu----GCCACUGUGGCgCUG- -5' |
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| 18566 | 3' | -53.4 | NC_004682.1 | + | 27547 | 0.71 | 0.526959 |
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Target: 5'- -gUCGCuaCGGCGGcgacacUGGCGCUGCGGCa -3' miRNA: 3'- caAGUGuaGCUGCC------ACUGUGGCGCUG- -5' |
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| 18566 | 3' | -53.4 | NC_004682.1 | + | 3221 | 0.71 | 0.495089 |
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Target: 5'- --cUACGUCGGCGGcgcGGCACaggGCGACa -3' miRNA: 3'- caaGUGUAGCUGCCa--CUGUGg--CGCUG- -5' |
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| 18566 | 3' | -53.4 | NC_004682.1 | + | 27656 | 0.71 | 0.484659 |
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Target: 5'- --cCACAUCGggcACGGUGAUuucgauggugAUCGCGGCg -3' miRNA: 3'- caaGUGUAGC---UGCCACUG----------UGGCGCUG- -5' |
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| 18566 | 3' | -53.4 | NC_004682.1 | + | 23156 | 0.71 | 0.484659 |
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Target: 5'- --cCACAgCGGCGG-GAuCGCCGCGAUc -3' miRNA: 3'- caaGUGUaGCUGCCaCU-GUGGCGCUG- -5' |
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| 18566 | 3' | -53.4 | NC_004682.1 | + | 34096 | 0.72 | 0.444052 |
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Target: 5'- -gUCgACGUUGGCGGUgaagGACACCGCGu- -3' miRNA: 3'- caAG-UGUAGCUGCCA----CUGUGGCGCug -5' |
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| 18566 | 3' | -53.4 | NC_004682.1 | + | 32110 | 0.72 | 0.434201 |
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Target: 5'- gGUUCACGUCGAa--UGACuCCGCGAUu -3' miRNA: 3'- -CAAGUGUAGCUgccACUGuGGCGCUG- -5' |
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| 18566 | 3' | -53.4 | NC_004682.1 | + | 1903 | 0.73 | 0.405427 |
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Target: 5'- --cCACAUCGACGGccagGugGCCGacuaCGACg -3' miRNA: 3'- caaGUGUAGCUGCCa---CugUGGC----GCUG- -5' |
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| 18566 | 3' | -53.4 | NC_004682.1 | + | 40290 | 0.74 | 0.368987 |
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Target: 5'- -gUCAUcucuUCGACGGUGugACCGaaCGGCa -3' miRNA: 3'- caAGUGu---AGCUGCCACugUGGC--GCUG- -5' |
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| 18566 | 3' | -53.4 | NC_004682.1 | + | 34573 | 1.08 | 0.001618 |
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Target: 5'- uGUUCACAUCGACGGUGACACCGCGACc -3' miRNA: 3'- -CAAGUGUAGCUGCCACUGUGGCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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