Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
18571 | 3' | -51.5 | NC_004682.1 | + | 8715 | 0.66 | 0.911851 |
Target: 5'- cUCAUCGCGAGGGggaCGaagUGAucuaccuGCUGGc -3' miRNA: 3'- -AGUAGCGCUCCUagaGCa--GCU-------UGACC- -5' |
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18571 | 3' | -51.5 | NC_004682.1 | + | 20261 | 0.66 | 0.908561 |
Target: 5'- cCcgCGUGuGGAgcaggUCUCGUCGGAgaacggcagccagcuCUGGg -3' miRNA: 3'- aGuaGCGCuCCU-----AGAGCAGCUU---------------GACC- -5' |
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18571 | 3' | -51.5 | NC_004682.1 | + | 21217 | 0.67 | 0.86866 |
Target: 5'- cCGUU-CGAGGAUCUgCGcggCGAGCUGa -3' miRNA: 3'- aGUAGcGCUCCUAGA-GCa--GCUUGACc -5' |
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18571 | 3' | -51.5 | NC_004682.1 | + | 30184 | 0.67 | 0.851954 |
Target: 5'- cCGaCGCcAGGAUCUCGUCG---UGGa -3' miRNA: 3'- aGUaGCGcUCCUAGAGCAGCuugACC- -5' |
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18571 | 3' | -51.5 | NC_004682.1 | + | 21685 | 0.67 | 0.84324 |
Target: 5'- uUCG-CGCGAgugucGGAUCgcCGaacUCGAACUGGa -3' miRNA: 3'- -AGUaGCGCU-----CCUAGa-GC---AGCUUGACC- -5' |
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18571 | 3' | -51.5 | NC_004682.1 | + | 31263 | 0.68 | 0.834297 |
Target: 5'- ---cUGCGAGGAUCggGUCcaugcccgaguaGAGCUGGu -3' miRNA: 3'- aguaGCGCUCCUAGagCAG------------CUUGACC- -5' |
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18571 | 3' | -51.5 | NC_004682.1 | + | 24843 | 0.69 | 0.790492 |
Target: 5'- cUCAUCGCGAcggcugaggacgagcGGAUCUacuUCGAGC-GGa -3' miRNA: 3'- -AGUAGCGCU---------------CCUAGAgc-AGCUUGaCC- -5' |
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18571 | 3' | -51.5 | NC_004682.1 | + | 42417 | 0.7 | 0.723812 |
Target: 5'- -gGUUGcCGGGGGUCgCGUUGAcCUGGa -3' miRNA: 3'- agUAGC-GCUCCUAGaGCAGCUuGACC- -5' |
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18571 | 3' | -51.5 | NC_004682.1 | + | 45673 | 0.7 | 0.723812 |
Target: 5'- gCAUCGuCGgcAGGAUCUCG-CGGGCcuUGGc -3' miRNA: 3'- aGUAGC-GC--UCCUAGAGCaGCUUG--ACC- -5' |
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18571 | 3' | -51.5 | NC_004682.1 | + | 14311 | 0.71 | 0.635387 |
Target: 5'- aCGggCGUGAGGGUCUCGUUGAGa--- -3' miRNA: 3'- aGUa-GCGCUCCUAGAGCAGCUUgacc -5' |
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18571 | 3' | -51.5 | NC_004682.1 | + | 25882 | 0.72 | 0.579647 |
Target: 5'- cUCGUCgGCGuuGGUCUUGUCGGucucCUGGg -3' miRNA: 3'- -AGUAG-CGCucCUAGAGCAGCUu---GACC- -5' |
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18571 | 3' | -51.5 | NC_004682.1 | + | 33805 | 0.95 | 0.023006 |
Target: 5'- aUCAUCGCGAGGAUCUgcUCGAACUGGg -3' miRNA: 3'- -AGUAGCGCUCCUAGAgcAGCUUGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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