Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18572 | 5' | -48.5 | NC_004682.1 | + | 12954 | 0.66 | 0.976718 |
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Target: 5'- aCGUC-CGUUuGGCUGAuGUCgGCGGAUg -3' miRNA: 3'- gGUAGaGCAAcUUGACU-CAG-CGCUUG- -5' |
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| 18572 | 5' | -48.5 | NC_004682.1 | + | 12173 | 0.66 | 0.976718 |
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Target: 5'- gCCAUCcCGUaccUGcGCUG-GUCGCagGAGCa -3' miRNA: 3'- -GGUAGaGCA---ACuUGACuCAGCG--CUUG- -5' |
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| 18572 | 5' | -48.5 | NC_004682.1 | + | 34381 | 0.67 | 0.955681 |
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Target: 5'- gCAUCUCGUUGAccgACuUGAGguagGCGuACg -3' miRNA: 3'- gGUAGAGCAACU---UG-ACUCag--CGCuUG- -5' |
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| 18572 | 5' | -48.5 | NC_004682.1 | + | 12760 | 0.67 | 0.951214 |
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Target: 5'- gCgAUCUCGUUGA----GGUCGCGGu- -3' miRNA: 3'- -GgUAGAGCAACUugacUCAGCGCUug -5' |
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| 18572 | 5' | -48.5 | NC_004682.1 | + | 31991 | 0.68 | 0.928639 |
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Target: 5'- gCCGUCUUGUUcaGAaggucgucguacucACUGccGGUCGCGGAg -3' miRNA: 3'- -GGUAGAGCAA--CU--------------UGAC--UCAGCGCUUg -5' |
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| 18572 | 5' | -48.5 | NC_004682.1 | + | 42702 | 0.74 | 0.658784 |
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Target: 5'- gCGUCUUGgcGAACcGGGUCGCGGc- -3' miRNA: 3'- gGUAGAGCaaCUUGaCUCAGCGCUug -5' |
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| 18572 | 5' | -48.5 | NC_004682.1 | + | 33541 | 1.14 | 0.002328 |
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Target: 5'- aCCAUCUCGUUGAACUGAGUCGCGAACg -3' miRNA: 3'- -GGUAGAGCAACUUGACUCAGCGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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