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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18577 | 3' | -59.2 | NC_004682.1 | + | 31259 | 0.66 | 0.414839 |
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Target: 5'- cGGGCUgcgaggaucgGGUCCAUGCCCGaguagagcugguagUaGGGGUu -3' miRNA: 3'- uCCCGGa---------UCAGGUACGGGC--------------A-CCCUAu -5' |
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| 18577 | 3' | -59.2 | NC_004682.1 | + | 29341 | 0.68 | 0.350256 |
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Target: 5'- aGGGGCCUuccucgaacacgaUCCAcgggGCCUGUGGGGa- -3' miRNA: 3'- -UCCCGGAuc-----------AGGUa---CGGGCACCCUau -5' |
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| 18577 | 3' | -59.2 | NC_004682.1 | + | 36775 | 0.69 | 0.299781 |
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Target: 5'- gAGGGCCUuGUCCAccgUGCCCG-GaGAUu -3' miRNA: 3'- -UCCCGGAuCAGGU---ACGGGCaCcCUAu -5' |
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| 18577 | 3' | -59.2 | NC_004682.1 | + | 38210 | 0.69 | 0.278129 |
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Target: 5'- gGGGGCuCUAGgccCCcgGCCCGUucucugucaaGGGAg- -3' miRNA: 3'- -UCCCG-GAUCa--GGuaCGGGCA----------CCCUau -5' |
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| 18577 | 3' | -59.2 | NC_004682.1 | + | 30421 | 1.06 | 0.000509 |
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Target: 5'- cAGGGCCUAGUCCAUGCCCGUGGGAUAc -3' miRNA: 3'- -UCCCGGAUCAGGUACGGGCACCCUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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