Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18579 | 5' | -56.1 | NC_004682.1 | + | 15104 | 0.66 | 0.651299 |
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Target: 5'- -cCCCGGUGaGcgcGCCGAUCUgauccggcugaUGGAGGa -3' miRNA: 3'- caGGGUCGC-Cau-CGGCUAGA-----------ACCUCU- -5' |
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| 18579 | 5' | -56.1 | NC_004682.1 | + | 50477 | 0.66 | 0.640235 |
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Target: 5'- cUCCCGGaGGggAGCCG--CUUGGGGGc -3' miRNA: 3'- cAGGGUCgCCa-UCGGCuaGAACCUCU- -5' |
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| 18579 | 5' | -56.1 | NC_004682.1 | + | 21796 | 0.66 | 0.638022 |
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Target: 5'- cUCCguGCGGUcggcaugccgcuGCCGAUCccgcugcUGGAGAc -3' miRNA: 3'- cAGGguCGCCAu-----------CGGCUAGa------ACCUCU- -5' |
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| 18579 | 5' | -56.1 | NC_004682.1 | + | 42918 | 0.66 | 0.629165 |
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Target: 5'- -gCCUAGCuGGUAGCCcucggGAUCUUcccucugcgaGGGGAu -3' miRNA: 3'- caGGGUCG-CCAUCGG-----CUAGAA----------CCUCU- -5' |
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| 18579 | 5' | -56.1 | NC_004682.1 | + | 33578 | 0.66 | 0.618097 |
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Target: 5'- -aCCCGGacaGGUAGCCGAaggCgucGGAGc -3' miRNA: 3'- caGGGUCg--CCAUCGGCUa--Gaa-CCUCu -5' |
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| 18579 | 5' | -56.1 | NC_004682.1 | + | 26924 | 0.67 | 0.563129 |
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Target: 5'- -gCCCAucGCGGcAGCCGcGUCcUGGGGGu -3' miRNA: 3'- caGGGU--CGCCaUCGGC-UAGaACCUCU- -5' |
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| 18579 | 5' | -56.1 | NC_004682.1 | + | 43498 | 0.68 | 0.530753 |
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Target: 5'- cGUCaCCGGgGGUGuuGCCGGUCUUGuAGu -3' miRNA: 3'- -CAG-GGUCgCCAU--CGGCUAGAACcUCu -5' |
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| 18579 | 5' | -56.1 | NC_004682.1 | + | 3555 | 0.68 | 0.49908 |
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Target: 5'- aGUCCUGGCGcUGGCCGA----GGAGAu -3' miRNA: 3'- -CAGGGUCGCcAUCGGCUagaaCCUCU- -5' |
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| 18579 | 5' | -56.1 | NC_004682.1 | + | 28739 | 1.08 | 0.000816 |
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Target: 5'- uGUCCCAGCGGUAGCCGAUCUUGGAGAc -3' miRNA: 3'- -CAGGGUCGCCAUCGGCUAGAACCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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