Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18580 | 5' | -53.8 | NC_004682.1 | + | 46559 | 0.66 | 0.797245 |
Target: 5'- uGAUCGgCAGaagCGCcUGGGCGAUCa -3' miRNA: 3'- cCUAGCaGUCcuaGCGcAUCCGCUGG- -5' |
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18580 | 5' | -53.8 | NC_004682.1 | + | 30866 | 0.66 | 0.81621 |
Target: 5'- ---cCGUCGcGGcaGUCGCGUugcGGGCGAUg -3' miRNA: 3'- ccuaGCAGU-CC--UAGCGCA---UCCGCUGg -5' |
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18580 | 5' | -53.8 | NC_004682.1 | + | 42415 | 0.66 | 0.825409 |
Target: 5'- cGGGUUGcCgGGGGUCGCGU---UGACCu -3' miRNA: 3'- -CCUAGCaG-UCCUAGCGCAuccGCUGG- -5' |
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18580 | 5' | -53.8 | NC_004682.1 | + | 27766 | 1.15 | 0.000638 |
Target: 5'- gGGAUCGUCAGGAUCGCGUAGGCGACCa -3' miRNA: 3'- -CCUAGCAGUCCUAGCGCAUCCGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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