Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18580 | 5' | -53.8 | NC_004682.1 | + | 36335 | 0.72 | 0.458674 |
Target: 5'- gGGGUUaUCAGGAUCuCgGUGGGCGAaCCc -3' miRNA: 3'- -CCUAGcAGUCCUAGcG-CAUCCGCU-GG- -5' |
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18580 | 5' | -53.8 | NC_004682.1 | + | 47530 | 0.74 | 0.347944 |
Target: 5'- uGGGUCGaCGGGGU-GCGUgcccaucAGGUGGCCg -3' miRNA: 3'- -CCUAGCaGUCCUAgCGCA-------UCCGCUGG- -5' |
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18580 | 5' | -53.8 | NC_004682.1 | + | 19495 | 0.78 | 0.227532 |
Target: 5'- cGGGUCGUCAuGGucugcAUCGC--AGGCGACCc -3' miRNA: 3'- -CCUAGCAGU-CC-----UAGCGcaUCCGCUGG- -5' |
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18580 | 5' | -53.8 | NC_004682.1 | + | 27766 | 1.15 | 0.000638 |
Target: 5'- gGGAUCGUCAGGAUCGCGUAGGCGACCa -3' miRNA: 3'- -CCUAGCAGUCCUAGCGCAUCCGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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