Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18582 | 5' | -53.3 | NC_004682.1 | + | 12991 | 0.66 | 0.831051 |
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Target: 5'- gGCCaGCCGaucaacgacaUCGACGUGGccaacCUGAUGAu -3' miRNA: 3'- -CGG-CGGCa---------AGCUGCAUCuc---GACUACU- -5' |
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| 18582 | 5' | -53.3 | NC_004682.1 | + | 37241 | 0.66 | 0.793019 |
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Target: 5'- -aCGCCGUgcUCGGCGUcccaGGAGCgcggGAa -3' miRNA: 3'- cgGCGGCA--AGCUGCA----UCUCGacuaCU- -5' |
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| 18582 | 5' | -53.3 | NC_004682.1 | + | 5847 | 0.66 | 0.793019 |
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Target: 5'- cGCCuacCUGUUCGACGUGccacuGGCUGuUGAg -3' miRNA: 3'- -CGGc--GGCAAGCUGCAUc----UCGACuACU- -5' |
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| 18582 | 5' | -53.3 | NC_004682.1 | + | 27670 | 0.67 | 0.752236 |
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Target: 5'- uGCCGgaUGUUCGAguCGUGGucGUUGGUGAu -3' miRNA: 3'- -CGGCg-GCAAGCU--GCAUCu-CGACUACU- -5' |
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| 18582 | 5' | -53.3 | NC_004682.1 | + | 30932 | 0.68 | 0.72029 |
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Target: 5'- uGCCGCCGUcggaucgcCGACG-AG-GCUGAUc- -3' miRNA: 3'- -CGGCGGCAa-------GCUGCaUCuCGACUAcu -5' |
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| 18582 | 5' | -53.3 | NC_004682.1 | + | 7995 | 0.69 | 0.609716 |
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Target: 5'- gGCUGCCGgUCGACG-AGAucCUGAUGu -3' miRNA: 3'- -CGGCGGCaAGCUGCaUCUc-GACUACu -5' |
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| 18582 | 5' | -53.3 | NC_004682.1 | + | 37643 | 0.7 | 0.587508 |
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Target: 5'- cCCGCCGgUCGugGaacgGGAucugcgugucGCUGAUGAc -3' miRNA: 3'- cGGCGGCaAGCugCa---UCU----------CGACUACU- -5' |
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| 18582 | 5' | -53.3 | NC_004682.1 | + | 12646 | 0.73 | 0.410785 |
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Target: 5'- -gCGCUGUUCGACGUAGAcgaacucgGCUGGa-- -3' miRNA: 3'- cgGCGGCAAGCUGCAUCU--------CGACUacu -5' |
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| 18582 | 5' | -53.3 | NC_004682.1 | + | 9514 | 0.79 | 0.186742 |
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Target: 5'- gGCUGCUGUUCGGCGUcaagggcgaGGAGCUGGg-- -3' miRNA: 3'- -CGGCGGCAAGCUGCA---------UCUCGACUacu -5' |
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| 18582 | 5' | -53.3 | NC_004682.1 | + | 26290 | 1.11 | 0.001091 |
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Target: 5'- uGCCGCCGUUCGACGUAGAGCUGAUGAa -3' miRNA: 3'- -CGGCGGCAAGCUGCAUCUCGACUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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