Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
18584 | 5' | -56.3 | NC_004682.1 | + | 26264 | 0.66 | 0.5889 |
Target: 5'- uCCGGUGGguGaaCUGCUGGGcAAGAg -3' miRNA: 3'- cGGCCACCguCacGACGGCUUcUUCU- -5' |
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18584 | 5' | -56.3 | NC_004682.1 | + | 9135 | 0.67 | 0.556157 |
Target: 5'- aGUCGGccagGGCGGUGCgugagGgCGAccuGGggGAc -3' miRNA: 3'- -CGGCCa---CCGUCACGa----CgGCU---UCuuCU- -5' |
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18584 | 5' | -56.3 | NC_004682.1 | + | 7706 | 0.68 | 0.513385 |
Target: 5'- aCCGGagcgUGGCg--GCUGCCGAccgaccAGAAGGa -3' miRNA: 3'- cGGCC----ACCGucaCGACGGCU------UCUUCU- -5' |
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18584 | 5' | -56.3 | NC_004682.1 | + | 34003 | 0.68 | 0.492504 |
Target: 5'- gGCCGaUGGCgGGUGCaGCCGccacGAAGAc -3' miRNA: 3'- -CGGCcACCG-UCACGaCGGCuu--CUUCU- -5' |
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18584 | 5' | -56.3 | NC_004682.1 | + | 20120 | 0.76 | 0.1609 |
Target: 5'- gGCUGGUgcGGCGGUGCUuccugGCCGAcGAGGGc -3' miRNA: 3'- -CGGCCA--CCGUCACGA-----CGGCUuCUUCU- -5' |
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18584 | 5' | -56.3 | NC_004682.1 | + | 25277 | 1.11 | 0.000515 |
Target: 5'- aGCCGGUGGCAGUGCUGCCGAAGAAGAc -3' miRNA: 3'- -CGGCCACCGUCACGACGGCUUCUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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