Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18586 | 3' | -65.1 | NC_004682.1 | + | 42887 | 0.66 | 0.261702 |
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Target: 5'- uCUCGGGGUagaagcUGcGCCACUGCucuGGCCc- -3' miRNA: 3'- -GAGCCCCA------GC-CGGUGACGcc-CCGGac -5' |
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| 18586 | 3' | -65.1 | NC_004682.1 | + | 33494 | 0.66 | 0.231601 |
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Target: 5'- -cCGccagCGGCCuGCUGCGGGGCCa- -3' miRNA: 3'- gaGCcccaGCCGG-UGACGCCCCGGac -5' |
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| 18586 | 3' | -65.1 | NC_004682.1 | + | 8825 | 0.67 | 0.204472 |
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Target: 5'- uCUCGcuGGGcaucuucaUCGGacugaUCACUGCGGGGCgCUGa -3' miRNA: 3'- -GAGC--CCC--------AGCC-----GGUGACGCCCCG-GAC- -5' |
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| 18586 | 3' | -65.1 | NC_004682.1 | + | 8970 | 0.68 | 0.184792 |
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Target: 5'- gCUUGaGGGcuUCGGCUcg-GCGGGGCCg- -3' miRNA: 3'- -GAGC-CCC--AGCCGGugaCGCCCCGGac -5' |
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| 18586 | 3' | -65.1 | NC_004682.1 | + | 45440 | 0.68 | 0.175592 |
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Target: 5'- gUCGGGGUCGGUCACcagGCcGcGGUCg- -3' miRNA: 3'- gAGCCCCAGCCGGUGa--CGcC-CCGGac -5' |
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| 18586 | 3' | -65.1 | NC_004682.1 | + | 23658 | 0.68 | 0.171147 |
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Target: 5'- -aCGGGccugCGGCCcucCUGCGGGGCgaGu -3' miRNA: 3'- gaGCCCca--GCCGGu--GACGCCCCGgaC- -5' |
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| 18586 | 3' | -65.1 | NC_004682.1 | + | 42475 | 0.7 | 0.121926 |
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Target: 5'- -cCGGGGUCGGCCuccCUGCGGccaCCUc -3' miRNA: 3'- gaGCCCCAGCCGGu--GACGCCcc-GGAc -5' |
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| 18586 | 3' | -65.1 | NC_004682.1 | + | 24539 | 1.07 | 0.000166 |
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Target: 5'- gCUCGGGGUCGGCCACUGCGGGGCCUGu -3' miRNA: 3'- -GAGCCCCAGCCGGUGACGCCCCGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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