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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18588 | 5' | -51.9 | NC_004682.1 | + | 29844 | 0.66 | 0.862126 |
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Target: 5'- gUGGGGUCGAgcgGGUuGUCcgGGAGCg--UCc -3' miRNA: 3'- -ACCUCAGCUa--CUA-CAG--CCUCGacaAG- -5' |
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| 18588 | 5' | -51.9 | NC_004682.1 | + | 32510 | 0.67 | 0.787129 |
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Target: 5'- aGGAGUaGggGGUGUCGGGGaUGUc- -3' miRNA: 3'- aCCUCAgCuaCUACAGCCUCgACAag -5' |
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| 18588 | 5' | -51.9 | NC_004682.1 | + | 46396 | 0.68 | 0.780987 |
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Target: 5'- aUGGGGUCGAUGAucuggucaucgagcgUGcCGGGGaUGUagUCg -3' miRNA: 3'- -ACCUCAGCUACU---------------ACaGCCUCgACA--AG- -5' |
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| 18588 | 5' | -51.9 | NC_004682.1 | + | 16700 | 0.69 | 0.712314 |
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Target: 5'- cUGGAGggcUCGGgucGAUGUCccGGCUGUUCu -3' miRNA: 3'- -ACCUC---AGCUa--CUACAGccUCGACAAG- -5' |
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| 18588 | 5' | -51.9 | NC_004682.1 | + | 15276 | 0.72 | 0.522256 |
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Target: 5'- aUGGAGggCGgcGGUGUCGGGGCcaacuacagcaaccgGUUCa -3' miRNA: 3'- -ACCUCa-GCuaCUACAGCCUCGa--------------CAAG- -5' |
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| 18588 | 5' | -51.9 | NC_004682.1 | + | 23952 | 1.11 | 0.00138 |
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Target: 5'- gUGGAGUCGAUGAUGUCGGAGCUGUUCa -3' miRNA: 3'- -ACCUCAGCUACUACAGCCUCGACAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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