Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18589 | 3' | -52.8 | NC_004682.1 | + | 42519 | 0.7 | 0.533171 |
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Target: 5'- aUC-AGGugCUCGAUGUacccgacguggCUCGGCgGCg -3' miRNA: 3'- aAGuUUCugGAGCUACA-----------GAGCCGgUG- -5' |
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| 18589 | 3' | -52.8 | NC_004682.1 | + | 46207 | 0.71 | 0.479135 |
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Target: 5'- cUCGAuGGCCUCGA--UCUCGGCaGCg -3' miRNA: 3'- aAGUUuCUGGAGCUacAGAGCCGgUG- -5' |
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| 18589 | 3' | -52.8 | NC_004682.1 | + | 32599 | 0.71 | 0.479135 |
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Target: 5'- gUCGAuaccGGCCUCGAUGcCUCGGgCGa -3' miRNA: 3'- aAGUUu---CUGGAGCUACaGAGCCgGUg -5' |
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| 18589 | 3' | -52.8 | NC_004682.1 | + | 27209 | 0.73 | 0.370966 |
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Target: 5'- gUCAGGGugU-CGAUGaUCUUGGCCACc -3' miRNA: 3'- aAGUUUCugGaGCUAC-AGAGCCGGUG- -5' |
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| 18589 | 3' | -52.8 | NC_004682.1 | + | 45307 | 0.74 | 0.335975 |
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Target: 5'- gUCGcGAGACCgucUCGAUGUCgcggCGGUCGCa -3' miRNA: 3'- aAGU-UUCUGG---AGCUACAGa---GCCGGUG- -5' |
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| 18589 | 3' | -52.8 | NC_004682.1 | + | 13661 | 0.74 | 0.327612 |
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Target: 5'- gUUCAu-GACCUCGGUgGUCaUgGGCCACa -3' miRNA: 3'- -AAGUuuCUGGAGCUA-CAG-AgCCGGUG- -5' |
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| 18589 | 3' | -52.8 | NC_004682.1 | + | 38799 | 0.75 | 0.288125 |
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Target: 5'- -----cGACCUUGGUcGUCUCGGCCAg -3' miRNA: 3'- aaguuuCUGGAGCUA-CAGAGCCGGUg -5' |
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| 18589 | 3' | -52.8 | NC_004682.1 | + | 23709 | 1.08 | 0.001531 |
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Target: 5'- gUUCAAAGACCUCGAUGUCUCGGCCACg -3' miRNA: 3'- -AAGUUUCUGGAGCUACAGAGCCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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