Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18590 | 5' | -56.2 | NC_004682.1 | + | 12486 | 0.66 | 0.67544 |
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Target: 5'- uCUCGcuguggcagcacaACAUgGUCGcUGUCCGUUGCGAa -3' miRNA: 3'- uGAGC-------------UGUG-CGGCaACAGGCAGCGCU- -5' |
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| 18590 | 5' | -56.2 | NC_004682.1 | + | 27568 | 0.66 | 0.642909 |
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Target: 5'- cGCUCGAUGCGCUcc-GUCCGcagcuccUCGCGc -3' miRNA: 3'- -UGAGCUGUGCGGcaaCAGGC-------AGCGCu -5' |
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| 18590 | 5' | -56.2 | NC_004682.1 | + | 28325 | 0.67 | 0.633121 |
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Target: 5'- cGCaUCGACccACGCCGcUGUCguaGUCGCu- -3' miRNA: 3'- -UG-AGCUG--UGCGGCaACAGg--CAGCGcu -5' |
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| 18590 | 5' | -56.2 | NC_004682.1 | + | 26603 | 0.68 | 0.568167 |
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Target: 5'- gUUCuuCGCGCCGUUGUCCaUCGgGu -3' miRNA: 3'- uGAGcuGUGCGGCAACAGGcAGCgCu -5' |
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| 18590 | 5' | -56.2 | NC_004682.1 | + | 45284 | 0.68 | 0.536263 |
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Target: 5'- gUUCGGguuCGucCCGUUGaUCCGUCGCGAg -3' miRNA: 3'- uGAGCUgu-GC--GGCAAC-AGGCAGCGCU- -5' |
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| 18590 | 5' | -56.2 | NC_004682.1 | + | 16367 | 0.7 | 0.445058 |
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Target: 5'- -gUCGACugGCCGUcucgaacuUCCGgCGCGAg -3' miRNA: 3'- ugAGCUGugCGGCAac------AGGCaGCGCU- -5' |
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| 18590 | 5' | -56.2 | NC_004682.1 | + | 46151 | 0.71 | 0.363199 |
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Target: 5'- uGCUCGAuCAgGCCGacguagucgGUgCCGUCGCGGu -3' miRNA: 3'- -UGAGCU-GUgCGGCaa-------CA-GGCAGCGCU- -5' |
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| 18590 | 5' | -56.2 | NC_004682.1 | + | 39709 | 0.71 | 0.363199 |
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Target: 5'- uCUCGAccuucggcucCugGCCuugGUCCGUCGCGGc -3' miRNA: 3'- uGAGCU----------GugCGGcaaCAGGCAGCGCU- -5' |
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| 18590 | 5' | -56.2 | NC_004682.1 | + | 23362 | 1.09 | 0.000934 |
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Target: 5'- aACUCGACACGCCGUUGUCCGUCGCGAg -3' miRNA: 3'- -UGAGCUGUGCGGCAACAGGCAGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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