Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18591 | 5' | -50.4 | NC_004682.1 | + | 23167 | 1.09 | 0.003101 |
Target: 5'- gGAUCGCCGCGAUCACCAUCGAAAUCAc -3' miRNA: 3'- -CUAGCGGCGCUAGUGGUAGCUUUAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 7435 | 0.74 | 0.492576 |
Target: 5'- --aCGCCGCgugGAUCACCAUCGccgccGUCAg -3' miRNA: 3'- cuaGCGGCG---CUAGUGGUAGCuu---UAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 33155 | 0.74 | 0.514219 |
Target: 5'- --gCGCCGUGGUCAgCGUCGugccGAUCAu -3' miRNA: 3'- cuaGCGGCGCUAGUgGUAGCu---UUAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 13968 | 0.72 | 0.615384 |
Target: 5'- --gCGCCaauGCGAUgGCCAUCGAGuUCGg -3' miRNA: 3'- cuaGCGG---CGCUAgUGGUAGCUUuAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 36559 | 0.72 | 0.642868 |
Target: 5'- -cUCGCCGCccugcggcuugcCGCCGUCGGAGUCGu -3' miRNA: 3'- cuAGCGGCGcua---------GUGGUAGCUUUAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 26677 | 0.71 | 0.661165 |
Target: 5'- -uUCGCCGCcauguccuccagGAUCGCCuugcccUCGGGAUCGg -3' miRNA: 3'- cuAGCGGCG------------CUAGUGGu-----AGCUUUAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 16263 | 0.71 | 0.683926 |
Target: 5'- uGGUCGCgGUG-UCACCGUCGAuGUgAa -3' miRNA: 3'- -CUAGCGgCGCuAGUGGUAGCUuUAgU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 12336 | 0.71 | 0.695232 |
Target: 5'- cGAUCaUCGCGAUCACCGaagggGAGAUCGa -3' miRNA: 3'- -CUAGcGGCGCUAGUGGUag---CUUUAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 23071 | 0.7 | 0.733103 |
Target: 5'- uGGUCGCCuacGCGAUCcugacgauccccgcaACCAUCGGugcGGUCGc -3' miRNA: 3'- -CUAGCGG---CGCUAG---------------UGGUAGCU---UUAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 19271 | 0.7 | 0.739669 |
Target: 5'- -uUCGgCGCGAcaUCACCuuugguGUCGAGAUCc -3' miRNA: 3'- cuAGCgGCGCU--AGUGG------UAGCUUUAGu -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 37891 | 0.7 | 0.739669 |
Target: 5'- --gCGCCGCGAUguCCG-CGAAGUUc -3' miRNA: 3'- cuaGCGGCGCUAguGGUaGCUUUAGu -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 19940 | 0.7 | 0.761234 |
Target: 5'- -cUCGCCgagGCGAUCACCGagCGAAGa-- -3' miRNA: 3'- cuAGCGG---CGCUAGUGGUa-GCUUUagu -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 39604 | 0.7 | 0.761234 |
Target: 5'- -uUCGUCGUGGUCAUCGUCacuccAGGUCAa -3' miRNA: 3'- cuAGCGGCGCUAGUGGUAGc----UUUAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 30679 | 0.7 | 0.761234 |
Target: 5'- --gUGuuGgGAUCGCCAUCGcuGUCGg -3' miRNA: 3'- cuaGCggCgCUAGUGGUAGCuuUAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 40348 | 0.69 | 0.812418 |
Target: 5'- cGUCGagCGCGGUCACCG-CGAAGccUCGc -3' miRNA: 3'- cUAGCg-GCGCUAGUGGUaGCUUU--AGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 37867 | 0.69 | 0.812418 |
Target: 5'- gGAUgCGCCGCuuGAUCAuCCGcUCGAcAUCAg -3' miRNA: 3'- -CUA-GCGGCG--CUAGU-GGU-AGCUuUAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 35740 | 0.68 | 0.822088 |
Target: 5'- aGAUCGgCGCGcUCACCGggGAGGUg- -3' miRNA: 3'- -CUAGCgGCGCuAGUGGUagCUUUAgu -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 31211 | 0.68 | 0.831542 |
Target: 5'- aGAUC-CUGCGAgaccgccggCACCAgCGGGAUCAg -3' miRNA: 3'- -CUAGcGGCGCUa--------GUGGUaGCUUUAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 47410 | 0.68 | 0.831542 |
Target: 5'- gGAUCGCCGgG---GCCGUCGAAccUCAg -3' miRNA: 3'- -CUAGCGGCgCuagUGGUAGCUUu-AGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 32439 | 0.68 | 0.840769 |
Target: 5'- -uUCGCCGUGAacgugaACCAUCGGGccGUCc -3' miRNA: 3'- cuAGCGGCGCUag----UGGUAGCUU--UAGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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