Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18591 | 5' | -50.4 | NC_004682.1 | + | 16263 | 0.71 | 0.683926 |
Target: 5'- uGGUCGCgGUG-UCACCGUCGAuGUgAa -3' miRNA: 3'- -CUAGCGgCGCuAGUGGUAGCUuUAgU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 26677 | 0.71 | 0.661165 |
Target: 5'- -uUCGCCGCcauguccuccagGAUCGCCuugcccUCGGGAUCGg -3' miRNA: 3'- cuAGCGGCG------------CUAGUGGu-----AGCUUUAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 36559 | 0.72 | 0.642868 |
Target: 5'- -cUCGCCGCccugcggcuugcCGCCGUCGGAGUCGu -3' miRNA: 3'- cuAGCGGCGcua---------GUGGUAGCUUUAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 13968 | 0.72 | 0.615384 |
Target: 5'- --gCGCCaauGCGAUgGCCAUCGAGuUCGg -3' miRNA: 3'- cuaGCGG---CGCUAgUGGUAGCUUuAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 33155 | 0.74 | 0.514219 |
Target: 5'- --gCGCCGUGGUCAgCGUCGugccGAUCAu -3' miRNA: 3'- cuaGCGGCGCUAGUgGUAGCu---UUAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 7435 | 0.74 | 0.492576 |
Target: 5'- --aCGCCGCgugGAUCACCAUCGccgccGUCAg -3' miRNA: 3'- cuaGCGGCG---CUAGUGGUAGCuu---UAGU- -5' |
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18591 | 5' | -50.4 | NC_004682.1 | + | 23167 | 1.09 | 0.003101 |
Target: 5'- gGAUCGCCGCGAUCACCAUCGAAAUCAc -3' miRNA: 3'- -CUAGCGGCGCUAGUGGUAGCUUUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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