Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18592 | 5' | -53.1 | NC_004682.1 | + | 14938 | 0.66 | 0.837188 |
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Target: 5'- --aGAAGGcgUUCCuGGUugUCAUCGUGGa -3' miRNA: 3'- ucaCUUCUa-GAGGcCCG--AGUAGCACC- -5' |
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| 18592 | 5' | -53.1 | NC_004682.1 | + | 37397 | 0.66 | 0.837188 |
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Target: 5'- gGGUGAGGuacUC-CCGaGGCcgCAggUCGUGGu -3' miRNA: 3'- -UCACUUCu--AGaGGC-CCGa-GU--AGCACC- -5' |
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| 18592 | 5' | -53.1 | NC_004682.1 | + | 16200 | 0.66 | 0.837188 |
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Target: 5'- aGGUGcuGGAUCgcaaCCGGcGCaUCggCGUGGg -3' miRNA: 3'- -UCACu-UCUAGa---GGCC-CG-AGuaGCACC- -5' |
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| 18592 | 5' | -53.1 | NC_004682.1 | + | 36084 | 0.67 | 0.793738 |
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Target: 5'- cGUcGAAGAugcugucaccguucaUCUUCGGGCUCcUCGUa- -3' miRNA: 3'- uCA-CUUCU---------------AGAGGCCCGAGuAGCAcc -5' |
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| 18592 | 5' | -53.1 | NC_004682.1 | + | 48595 | 0.67 | 0.789771 |
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Target: 5'- -uUGuuGuUCUCCGGGCaCcgCGUGGc -3' miRNA: 3'- ucACuuCuAGAGGCCCGaGuaGCACC- -5' |
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| 18592 | 5' | -53.1 | NC_004682.1 | + | 19075 | 0.67 | 0.789771 |
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Target: 5'- -cUGggGcUUccgcgacuucgUCCGGGCUCAUC-UGGg -3' miRNA: 3'- ucACuuCuAG-----------AGGCCCGAGUAGcACC- -5' |
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| 18592 | 5' | -53.1 | NC_004682.1 | + | 46074 | 0.68 | 0.727439 |
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Target: 5'- cAGUGcuGGUCUCCGcagucgucGGCUUcggCGUGGu -3' miRNA: 3'- -UCACuuCUAGAGGC--------CCGAGua-GCACC- -5' |
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| 18592 | 5' | -53.1 | NC_004682.1 | + | 6253 | 0.68 | 0.716637 |
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Target: 5'- cAGUGAcgccGAcCUCCGGGCUCugggCcUGGa -3' miRNA: 3'- -UCACUu---CUaGAGGCCCGAGua--GcACC- -5' |
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| 18592 | 5' | -53.1 | NC_004682.1 | + | 22837 | 1.12 | 0.001102 |
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Target: 5'- gAGUGAAGAUCUCCGGGCUCAUCGUGGa -3' miRNA: 3'- -UCACUUCUAGAGGCCCGAGUAGCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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