Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18596 | 5' | -57.8 | NC_004682.1 | + | 9166 | 0.66 | 0.564337 |
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Target: 5'- gGGGGaCG-CUGA-GGACGAGuUCCgCg -3' miRNA: 3'- -UCCC-GCaGACUaCCUGCUCuAGGgGa -5' |
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| 18596 | 5' | -57.8 | NC_004682.1 | + | 32460 | 0.66 | 0.553647 |
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Target: 5'- cGGGcCGUCccGAaGGACGAugguGAUCUCCUu -3' miRNA: 3'- uCCC-GCAGa-CUaCCUGCU----CUAGGGGA- -5' |
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| 18596 | 5' | -57.8 | NC_004682.1 | + | 29366 | 0.67 | 0.49102 |
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Target: 5'- cGGGGC--CUG-UGGG-GAGGUCCCCa -3' miRNA: 3'- -UCCCGcaGACuACCUgCUCUAGGGGa -5' |
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| 18596 | 5' | -57.8 | NC_004682.1 | + | 1843 | 0.68 | 0.451141 |
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Target: 5'- cGGGCGUCUGcu---CGGGcUCCCCg -3' miRNA: 3'- uCCCGCAGACuaccuGCUCuAGGGGa -5' |
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| 18596 | 5' | -57.8 | NC_004682.1 | + | 7158 | 0.68 | 0.430927 |
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Target: 5'- uGGGGCGUcCUGAacUGGugGgcugcguacgucaAGAcCCCCg -3' miRNA: 3'- -UCCCGCA-GACU--ACCugC-------------UCUaGGGGa -5' |
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| 18596 | 5' | -57.8 | NC_004682.1 | + | 15076 | 0.72 | 0.261855 |
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Target: 5'- -uGGCG-CUGGUGGACGAGcgcuaucaccUCCCCg -3' miRNA: 3'- ucCCGCaGACUACCUGCUCu---------AGGGGa -5' |
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| 18596 | 5' | -57.8 | NC_004682.1 | + | 21212 | 1.08 | 0.000623 |
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Target: 5'- cAGGGCGUCUGAUGGACGAGAUCCCCUg -3' miRNA: 3'- -UCCCGCAGACUACCUGCUCUAGGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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