Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18598 | 5' | -61.6 | NC_004682.1 | + | 24040 | 0.66 | 0.401588 |
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Target: 5'- aCUCGCaccGCGUGcuGCgGGGGAuGGacCGCUGg -3' miRNA: 3'- -GAGCGc--UGCAC--UGgCCCCU-CC--GCGAC- -5' |
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| 18598 | 5' | -61.6 | NC_004682.1 | + | 16326 | 0.68 | 0.270435 |
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Target: 5'- -cCGCGACGgcaugaagGACUGGGGccGCGCc- -3' miRNA: 3'- gaGCGCUGCa-------CUGGCCCCucCGCGac -5' |
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| 18598 | 5' | -61.6 | NC_004682.1 | + | 45736 | 0.69 | 0.263901 |
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Target: 5'- -gCGCGGCGUcGACCacGGGGGucGGUGCg- -3' miRNA: 3'- gaGCGCUGCA-CUGG--CCCCU--CCGCGac -5' |
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| 18598 | 5' | -61.6 | NC_004682.1 | + | 14322 | 0.69 | 0.248742 |
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Target: 5'- -aCGCGGCGguccucgaccuccGugCGGcucuGGAGGCGCUGc -3' miRNA: 3'- gaGCGCUGCa------------CugGCC----CCUCCGCGAC- -5' |
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| 18598 | 5' | -61.6 | NC_004682.1 | + | 15725 | 0.7 | 0.205452 |
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Target: 5'- -gUGCGugGUGGCCGGcGGuGuGCGCc- -3' miRNA: 3'- gaGCGCugCACUGGCC-CCuC-CGCGac -5' |
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| 18598 | 5' | -61.6 | NC_004682.1 | + | 20584 | 1.08 | 0.000314 |
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Target: 5'- uCUCGCGACGUGACCGGGGAGGCGCUGa -3' miRNA: 3'- -GAGCGCUGCACUGGCCCCUCCGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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