Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18602 | 3' | -59.4 | NC_004682.1 | + | 24148 | 0.66 | 0.44998 |
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Target: 5'- aUCCCgagGGCaAGGCGAucCUGgaGGacauGGCGg -3' miRNA: 3'- -AGGGa--CCG-UCCGCUu-GACgaCU----CCGU- -5' |
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| 18602 | 3' | -59.4 | NC_004682.1 | + | 4581 | 0.66 | 0.44998 |
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Target: 5'- gCUCgGGCGcuGGCGAGCUGacauggacGAGGCGa -3' miRNA: 3'- aGGGaCCGU--CCGCUUGACga------CUCCGU- -5' |
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| 18602 | 3' | -59.4 | NC_004682.1 | + | 24502 | 0.66 | 0.440262 |
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Target: 5'- aCCgCUGGCaucagccaggucGGcGCGGACUaccgggGCUGGGGCu -3' miRNA: 3'- aGG-GACCG------------UC-CGCUUGA------CGACUCCGu -5' |
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| 18602 | 3' | -59.4 | NC_004682.1 | + | 4434 | 0.66 | 0.440262 |
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Target: 5'- gCCUUGGUccucuaccgGGGCGAcaaggagcgcGCUGCUcuggcucuccgcGAGGCGu -3' miRNA: 3'- aGGGACCG---------UCCGCU----------UGACGA------------CUCCGU- -5' |
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| 18602 | 3' | -59.4 | NC_004682.1 | + | 28076 | 0.66 | 0.439297 |
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Target: 5'- cUCCCUcGGCgacgaagacgcucGGGCGuGGCUGCUcGcGGCGg -3' miRNA: 3'- -AGGGA-CCG-------------UCCGC-UUGACGA-CuCCGU- -5' |
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| 18602 | 3' | -59.4 | NC_004682.1 | + | 49017 | 0.67 | 0.402628 |
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Target: 5'- gCCC-GGaCGGGCGAACgcgGgUGAGGaCGc -3' miRNA: 3'- aGGGaCC-GUCCGCUUGa--CgACUCC-GU- -5' |
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| 18602 | 3' | -59.4 | NC_004682.1 | + | 10687 | 0.7 | 0.241136 |
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Target: 5'- gUCCUGGC-GGCGAuccugcucgauGCUGCcGcAGGCAa -3' miRNA: 3'- aGGGACCGuCCGCU-----------UGACGaC-UCCGU- -5' |
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| 18602 | 3' | -59.4 | NC_004682.1 | + | 26262 | 0.77 | 0.078299 |
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Target: 5'- cUUCC-GGUGGGUGAACUGCUG-GGCAa -3' miRNA: 3'- -AGGGaCCGUCCGCUUGACGACuCCGU- -5' |
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| 18602 | 3' | -59.4 | NC_004682.1 | + | 18272 | 1.09 | 0.000304 |
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Target: 5'- uUCCCUGGCAGGCGAACUGCUGAGGCAg -3' miRNA: 3'- -AGGGACCGUCCGCUUGACGACUCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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