Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18602 | 5' | -57 | NC_004682.1 | + | 35838 | 0.66 | 0.598605 |
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Target: 5'- gGAUCGCgucgUCCCggCGCACCgacGCCuuguGGGCGu -3' miRNA: 3'- -CUAGCG----AGGG--GUGUGGa--UGGu---CCUGU- -5' |
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| 18602 | 5' | -57 | NC_004682.1 | + | 14139 | 0.66 | 0.55396 |
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Target: 5'- aGAUCGCcagCCaggagcggacggaCUACGCCUACUacaAGGGCAg -3' miRNA: 3'- -CUAGCGa--GG-------------GGUGUGGAUGG---UCCUGU- -5' |
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| 18602 | 5' | -57 | NC_004682.1 | + | 5105 | 0.67 | 0.54428 |
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Target: 5'- cAUCgGCUUCagCCACACCUGgCAGGAg- -3' miRNA: 3'- cUAG-CGAGG--GGUGUGGAUgGUCCUgu -5' |
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| 18602 | 5' | -57 | NC_004682.1 | + | 15148 | 0.67 | 0.533592 |
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Target: 5'- aGAUC-CUCCCCgguggACGCC-ACCuguGGGCAu -3' miRNA: 3'- -CUAGcGAGGGG-----UGUGGaUGGu--CCUGU- -5' |
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| 18602 | 5' | -57 | NC_004682.1 | + | 48172 | 0.68 | 0.438589 |
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Target: 5'- aGGUCGCUgCCgCCACACCgggcgagcaucUggucccaugucucgACCAGGACGc -3' miRNA: 3'- -CUAGCGA-GG-GGUGUGG-----------A--------------UGGUCCUGU- -5' |
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| 18602 | 5' | -57 | NC_004682.1 | + | 10601 | 0.69 | 0.394384 |
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Target: 5'- --cCGCUCCCCGCAUCauuCgCGGGugGg -3' miRNA: 3'- cuaGCGAGGGGUGUGGau-G-GUCCugU- -5' |
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| 18602 | 5' | -57 | NC_004682.1 | + | 24201 | 0.69 | 0.394384 |
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Target: 5'- uGGUCGUUCaUCCGCAUCgcCCAGGAUc -3' miRNA: 3'- -CUAGCGAG-GGGUGUGGauGGUCCUGu -5' |
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| 18602 | 5' | -57 | NC_004682.1 | + | 48396 | 0.72 | 0.266991 |
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Target: 5'- cAUCGC-CCCCAUugCcgUGCCAGcGACAa -3' miRNA: 3'- cUAGCGaGGGGUGugG--AUGGUC-CUGU- -5' |
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| 18602 | 5' | -57 | NC_004682.1 | + | 18307 | 1.08 | 0.00065 |
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Target: 5'- aGAUCGCUCCCCACACCUACCAGGACAu -3' miRNA: 3'- -CUAGCGAGGGGUGUGGAUGGUCCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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