Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18605 | 3' | -55 | NC_004682.1 | + | 18269 | 0.65 | 0.709271 |
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Target: 5'- cCGUucccUGGCaGGCGAACUG-CUGAGg- -3' miRNA: 3'- aGCA----GCCGaCUGCUUGACcGACUCaa -5' |
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| 18605 | 3' | -55 | NC_004682.1 | + | 35196 | 0.66 | 0.680615 |
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Target: 5'- uUCGUCaaucaGGCgGGCGAGCgcucggcuucccccGGCUGGGUc -3' miRNA: 3'- -AGCAG-----CCGaCUGCUUGa-------------CCGACUCAa -5' |
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| 18605 | 3' | -55 | NC_004682.1 | + | 24519 | 0.67 | 0.642684 |
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Target: 5'- -gGUCGGC--GCGGACUaccggGGCUGGGg- -3' miRNA: 3'- agCAGCCGacUGCUUGA-----CCGACUCaa -5' |
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| 18605 | 3' | -55 | NC_004682.1 | + | 23189 | 0.68 | 0.552573 |
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Target: 5'- ---cCGGCacgacauUGACGAcCUGGCUGAGg- -3' miRNA: 3'- agcaGCCG-------ACUGCUuGACCGACUCaa -5' |
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| 18605 | 3' | -55 | NC_004682.1 | + | 5938 | 0.69 | 0.510422 |
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Target: 5'- cUCGaCGGCaagGGCGAgcGCUGGCUGGc-- -3' miRNA: 3'- -AGCaGCCGa--CUGCU--UGACCGACUcaa -5' |
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| 18605 | 3' | -55 | NC_004682.1 | + | 17733 | 1.06 | 0.001524 |
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Target: 5'- aUCGUCGGCUGACGAACUGGCUGAGUUc -3' miRNA: 3'- -AGCAGCCGACUGCUUGACCGACUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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