Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18606 | 3' | -63.9 | NC_004682.1 | + | 6085 | 0.66 | 0.302088 |
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Target: 5'- cGGcGGGCUGGuGagccgUUGGCagaagacgaugCUGGCCCg -3' miRNA: 3'- uCC-CCCGACC-Caa---AGCCG-----------GACCGGGg -5' |
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| 18606 | 3' | -63.9 | NC_004682.1 | + | 42457 | 0.66 | 0.267255 |
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Target: 5'- uGGGGaugaacuGGCUGgccGGggUCGGCCUcccugcGGCCaCCu -3' miRNA: 3'- -UCCC-------CCGAC---CCaaAGCCGGA------CCGG-GG- -5' |
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| 18606 | 3' | -63.9 | NC_004682.1 | + | 15182 | 0.67 | 0.248324 |
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Target: 5'- uGGGGGGagGGGUUUCcuuggcgGGCCUu-CCCCu -3' miRNA: 3'- -UCCCCCgaCCCAAAG-------CCGGAccGGGG- -5' |
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| 18606 | 3' | -63.9 | NC_004682.1 | + | 43256 | 0.67 | 0.236328 |
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Target: 5'- cGGGGGacgaGGUUgCGGCCUcgaugcgcccgccGGCCUCg -3' miRNA: 3'- uCCCCCgac-CCAAaGCCGGA-------------CCGGGG- -5' |
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| 18606 | 3' | -63.9 | NC_004682.1 | + | 22981 | 0.68 | 0.198634 |
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Target: 5'- --cGGGCUGGccUUCGGgCUGGUCCg -3' miRNA: 3'- uccCCCGACCcaAAGCCgGACCGGGg -5' |
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| 18606 | 3' | -63.9 | NC_004682.1 | + | 17608 | 1.1 | 0.000119 |
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Target: 5'- gAGGGGGCUGGGUUUCGGCCUGGCCCCc -3' miRNA: 3'- -UCCCCCGACCCAAAGCCGGACCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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