Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18609 | 3' | -48.4 | NC_004682.1 | + | 20600 | 0.67 | 0.959151 |
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Target: 5'- gGGAGGCgcuGAUCcggCUGCaugagGCCGGGUUCAc -3' miRNA: 3'- gCUUCUG---CUAGa--GAUG-----CGGUUCAAGU- -5' |
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| 18609 | 3' | -48.4 | NC_004682.1 | + | 18939 | 0.68 | 0.934916 |
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Target: 5'- gCGAcGGCGcgCUCUcucagGCGCCAAGg--- -3' miRNA: 3'- -GCUuCUGCuaGAGA-----UGCGGUUCaagu -5' |
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| 18609 | 3' | -48.4 | NC_004682.1 | + | 39745 | 0.69 | 0.903106 |
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Target: 5'- gCGAAGACGAUCaccUCaGCGgCCA-GUUCc -3' miRNA: 3'- -GCUUCUGCUAG---AGaUGC-GGUuCAAGu -5' |
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| 18609 | 3' | -48.4 | NC_004682.1 | + | 15462 | 0.69 | 0.895837 |
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Target: 5'- aGAGGACGAcCUCgACGCCA-GUg-- -3' miRNA: 3'- gCUUCUGCUaGAGaUGCGGUuCAagu -5' |
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| 18609 | 3' | -48.4 | NC_004682.1 | + | 29514 | 0.69 | 0.895837 |
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Target: 5'- cCGAAGGCGGUCUCacUGCcccagcCCGAGUUg- -3' miRNA: 3'- -GCUUCUGCUAGAG--AUGc-----GGUUCAAgu -5' |
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| 18609 | 3' | -48.4 | NC_004682.1 | + | 14750 | 0.71 | 0.79805 |
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Target: 5'- cCGAAGAUGAacggugacagcaUCUUcgACGCgAAGUUCAa -3' miRNA: 3'- -GCUUCUGCU------------AGAGa-UGCGgUUCAAGU- -5' |
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| 18609 | 3' | -48.4 | NC_004682.1 | + | 6108 | 0.71 | 0.791912 |
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Target: 5'- aGAAGACGAUgCUggcccgcuacgcggcCUACGCCAAGg--- -3' miRNA: 3'- gCUUCUGCUA-GA---------------GAUGCGGUUCaagu -5' |
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| 18609 | 3' | -48.4 | NC_004682.1 | + | 16557 | 1.09 | 0.004715 |
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Target: 5'- uCGAAGACGAUCUCUACGCCAAGUUCAc -3' miRNA: 3'- -GCUUCUGCUAGAGAUGCGGUUCAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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