Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18609 | 5' | -58 | NC_004682.1 | + | 3131 | 0.69 | 0.407603 |
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Target: 5'- gUCGAUCaCUGGCGCAGGcgGCGCagcagccgccaaCUCGg -3' miRNA: 3'- gAGCUAG-GGCUGCGUCC--UGUGg-----------GAGC- -5' |
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| 18609 | 5' | -58 | NC_004682.1 | + | 9439 | 0.69 | 0.381858 |
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Target: 5'- gCUCGGUCaCCGACGCAgcugcccGGACGCUg--- -3' miRNA: 3'- -GAGCUAG-GGCUGCGU-------CCUGUGGgagc -5' |
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| 18609 | 5' | -58 | NC_004682.1 | + | 16145 | 0.7 | 0.35722 |
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Target: 5'- aCUCGGuUCCUGAUGCGGGcgACGuuCUCGc -3' miRNA: 3'- -GAGCU-AGGGCUGCGUCC--UGUggGAGC- -5' |
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| 18609 | 5' | -58 | NC_004682.1 | + | 14056 | 0.7 | 0.34089 |
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Target: 5'- gCUCGaAUCuCCGggcACGguGGACaagGCCCUCGc -3' miRNA: 3'- -GAGC-UAG-GGC---UGCguCCUG---UGGGAGC- -5' |
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| 18609 | 5' | -58 | NC_004682.1 | + | 49647 | 0.72 | 0.267459 |
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Target: 5'- gCUCGAUcucaaguuggaCCCGGCgGCGGu-CACCCUCGg -3' miRNA: 3'- -GAGCUA-----------GGGCUG-CGUCcuGUGGGAGC- -5' |
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| 18609 | 5' | -58 | NC_004682.1 | + | 38149 | 0.74 | 0.191236 |
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Target: 5'- aCUCG-UCCCccuggugGACGUAgucguGGACGCCCUCGg -3' miRNA: 3'- -GAGCuAGGG-------CUGCGU-----CCUGUGGGAGC- -5' |
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| 18609 | 5' | -58 | NC_004682.1 | + | 43538 | 0.78 | 0.10885 |
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Target: 5'- cCUUGAgg-CGGCGCAGGAUGCCCUCGc -3' miRNA: 3'- -GAGCUaggGCUGCGUCCUGUGGGAGC- -5' |
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| 18609 | 5' | -58 | NC_004682.1 | + | 16593 | 1.08 | 0.000713 |
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Target: 5'- uCUCGAUCCCGACGCAGGACACCCUCGu -3' miRNA: 3'- -GAGCUAGGGCUGCGUCCUGUGGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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