miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
18610 5' -57.7 NC_004682.1 + 9573 0.67 0.506886
Target:  5'- aGAACCGUGGuGGCCG-GUcgGACCcguccGGGc -3'
miRNA:   3'- -CUUGGCACCcUCGGCaCG--UUGGa----CCC- -5'
18610 5' -57.7 NC_004682.1 + 48169 0.68 0.456601
Target:  5'- cGACCuucgGGGAcagcgcGUCGUagGCAGCCUGGGu -3'
miRNA:   3'- cUUGGca--CCCU------CGGCA--CGUUGGACCC- -5'
18610 5' -57.7 NC_004682.1 + 22993 0.69 0.408203
Target:  5'- cGGGCUGguccgGGGGGCCGUccuuuucGCGuuUCUGGGg -3'
miRNA:   3'- -CUUGGCa----CCCUCGGCA-------CGUu-GGACCC- -5'
18610 5' -57.7 NC_004682.1 + 30792 0.69 0.399999
Target:  5'- cGAGCCGUGcGAGCC-UGCAGC--GGGu -3'
miRNA:   3'- -CUUGGCACcCUCGGcACGUUGgaCCC- -5'
18610 5' -57.7 NC_004682.1 + 33415 0.7 0.33991
Target:  5'- --cCCGuUGGGGGCCuccugaGCcGCCUGGGg -3'
miRNA:   3'- cuuGGC-ACCCUCGGca----CGuUGGACCC- -5'
18610 5' -57.7 NC_004682.1 + 16039 1.09 0.000507
Target:  5'- cGAACCGUGGGAGCCGUGCAACCUGGGc -3'
miRNA:   3'- -CUUGGCACCCUCGGCACGUUGGACCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.