Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18610 | 5' | -57.7 | NC_004682.1 | + | 9573 | 0.67 | 0.506886 |
Target: 5'- aGAACCGUGGuGGCCG-GUcgGACCcguccGGGc -3' miRNA: 3'- -CUUGGCACCcUCGGCaCG--UUGGa----CCC- -5' |
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18610 | 5' | -57.7 | NC_004682.1 | + | 48169 | 0.68 | 0.456601 |
Target: 5'- cGACCuucgGGGAcagcgcGUCGUagGCAGCCUGGGu -3' miRNA: 3'- cUUGGca--CCCU------CGGCA--CGUUGGACCC- -5' |
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18610 | 5' | -57.7 | NC_004682.1 | + | 22993 | 0.69 | 0.408203 |
Target: 5'- cGGGCUGguccgGGGGGCCGUccuuuucGCGuuUCUGGGg -3' miRNA: 3'- -CUUGGCa----CCCUCGGCA-------CGUu-GGACCC- -5' |
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18610 | 5' | -57.7 | NC_004682.1 | + | 30792 | 0.69 | 0.399999 |
Target: 5'- cGAGCCGUGcGAGCC-UGCAGC--GGGu -3' miRNA: 3'- -CUUGGCACcCUCGGcACGUUGgaCCC- -5' |
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18610 | 5' | -57.7 | NC_004682.1 | + | 33415 | 0.7 | 0.33991 |
Target: 5'- --cCCGuUGGGGGCCuccugaGCcGCCUGGGg -3' miRNA: 3'- cuuGGC-ACCCUCGGca----CGuUGGACCC- -5' |
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18610 | 5' | -57.7 | NC_004682.1 | + | 16039 | 1.09 | 0.000507 |
Target: 5'- cGAACCGUGGGAGCCGUGCAACCUGGGc -3' miRNA: 3'- -CUUGGCACCCUCGGCACGUUGGACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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