Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18611 | 5' | -58.5 | NC_004682.1 | + | 16816 | 0.65 | 0.515991 |
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Target: 5'- -cGGUGGauUGcucAAGGGGGCaacgauuuuccccgAGGCGUCCa -3' miRNA: 3'- cuCCGCU--AC---UUCCUCCG--------------UCCGCAGGa -5' |
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| 18611 | 5' | -58.5 | NC_004682.1 | + | 29262 | 0.66 | 0.498353 |
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Target: 5'- -cGGCGAUGAAGucGGCAucGGCcucgcucuggagGUCCg -3' miRNA: 3'- cuCCGCUACUUCcuCCGU--CCG------------CAGGa -5' |
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| 18611 | 5' | -58.5 | NC_004682.1 | + | 23792 | 0.66 | 0.497323 |
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Target: 5'- cAGGCGGUGAAGGAcuGGUGGacccagaugaccaGCGgCCUg -3' miRNA: 3'- cUCCGCUACUUCCU--CCGUC-------------CGCaGGA- -5' |
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| 18611 | 5' | -58.5 | NC_004682.1 | + | 18526 | 0.66 | 0.488104 |
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Target: 5'- -cGGCGGUcGAGGAGGaCAccagcGGUGUCUa -3' miRNA: 3'- cuCCGCUAcUUCCUCC-GU-----CCGCAGGa -5' |
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| 18611 | 5' | -58.5 | NC_004682.1 | + | 13823 | 0.67 | 0.457977 |
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Target: 5'- cGAGGCGAaGGAGcGAGGCcggaAGGCcgaggCCa -3' miRNA: 3'- -CUCCGCUaCUUC-CUCCG----UCCGca---GGa -5' |
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| 18611 | 5' | -58.5 | NC_004682.1 | + | 35114 | 0.69 | 0.340307 |
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Target: 5'- aGAGGCGGgacucAGGGAGGUAGaUGUCCc -3' miRNA: 3'- -CUCCGCUac---UUCCUCCGUCcGCAGGa -5' |
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| 18611 | 5' | -58.5 | NC_004682.1 | + | 38050 | 0.71 | 0.259081 |
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Target: 5'- cGGGGgGAucgcuUGggGGAGGCcgguuucgGGGCGUCa- -3' miRNA: 3'- -CUCCgCU-----ACuuCCUCCG--------UCCGCAGga -5' |
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| 18611 | 5' | -58.5 | NC_004682.1 | + | 15386 | 1.08 | 0.000457 |
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Target: 5'- cGAGGCGAUGAAGGAGGCAGGCGUCCUg -3' miRNA: 3'- -CUCCGCUACUUCCUCCGUCCGCAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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