Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18613 | 5' | -58.2 | NC_004682.1 | + | 29290 | 0.66 | 0.497488 |
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Target: 5'- --cUGGAGGuCCgcAGCGAugagcuucCGGGUCUCc -3' miRNA: 3'- agaACCUCCuGG--UCGCUc-------GCCCAGAG- -5' |
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| 18613 | 5' | -58.2 | NC_004682.1 | + | 23657 | 0.67 | 0.487283 |
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Target: 5'- ---aGGA-GACCGGCGcgaAGUGGGUCg- -3' miRNA: 3'- agaaCCUcCUGGUCGC---UCGCCCAGag -5' |
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| 18613 | 5' | -58.2 | NC_004682.1 | + | 49448 | 0.68 | 0.418892 |
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Target: 5'- ----aGAGGACCAGCGAgucgcugucgagGCGGGUa-- -3' miRNA: 3'- agaacCUCCUGGUCGCU------------CGCCCAgag -5' |
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| 18613 | 5' | -58.2 | NC_004682.1 | + | 37368 | 0.69 | 0.339295 |
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Target: 5'- cCUUGGccGGGauGCCGGUcuuggauGGGCGGGUCUg -3' miRNA: 3'- aGAACC--UCC--UGGUCG-------CUCGCCCAGAg -5' |
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| 18613 | 5' | -58.2 | NC_004682.1 | + | 35084 | 0.69 | 0.335253 |
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Target: 5'- cCUUGauGAGGACCAGgGcgagcuugggggagaGGCGGGaCUCa -3' miRNA: 3'- aGAAC--CUCCUGGUCgC---------------UCGCCCaGAG- -5' |
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| 18613 | 5' | -58.2 | NC_004682.1 | + | 7490 | 0.7 | 0.301208 |
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Target: 5'- aCUUGGGGGAguggcCCcGCGAGaGGGUCg- -3' miRNA: 3'- aGAACCUCCU-----GGuCGCUCgCCCAGag -5' |
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| 18613 | 5' | -58.2 | NC_004682.1 | + | 21832 | 0.71 | 0.265833 |
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Target: 5'- --cUGGA-GACCAGCGAGaCGGGcCUg -3' miRNA: 3'- agaACCUcCUGGUCGCUC-GCCCaGAg -5' |
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| 18613 | 5' | -58.2 | NC_004682.1 | + | 14707 | 1.09 | 0.000454 |
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Target: 5'- aUCUUGGAGGACCAGCGAGCGGGUCUCc -3' miRNA: 3'- -AGAACCUCCUGGUCGCUCGCCCAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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