Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18620 | 3' | -57.5 | NC_004682.1 | + | 17390 | 0.7 | 0.310633 |
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Target: 5'- gGUGGugGcACCCAGAgGCAGUcgaaCGCGCc -3' miRNA: 3'- -UACUugC-UGGGUCU-CGUCGa---GCGCGa -5' |
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| 18620 | 3' | -57.5 | NC_004682.1 | + | 16690 | 0.7 | 0.302972 |
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Target: 5'- gGUGAugGGCCUGGAG-GGCUCGgGUc -3' miRNA: 3'- -UACUugCUGGGUCUCgUCGAGCgCGa -5' |
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| 18620 | 3' | -57.5 | NC_004682.1 | + | 26652 | 0.72 | 0.234295 |
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Target: 5'- gAUGAACGACCagccgucGAGCAGCUuCGcCGCc -3' miRNA: 3'- -UACUUGCUGGgu-----CUCGUCGA-GC-GCGa -5' |
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| 18620 | 3' | -57.5 | NC_004682.1 | + | 26910 | 0.74 | 0.16964 |
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Target: 5'- --cAGCGACCCGGAGCGGUccuugUCGCGgUa -3' miRNA: 3'- uacUUGCUGGGUCUCGUCG-----AGCGCgA- -5' |
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| 18620 | 3' | -57.5 | NC_004682.1 | + | 38554 | 0.76 | 0.132124 |
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Target: 5'- -gGGACGACCCAGGauGCGGCccucacggaUCGCGCc -3' miRNA: 3'- uaCUUGCUGGGUCU--CGUCG---------AGCGCGa -5' |
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| 18620 | 3' | -57.5 | NC_004682.1 | + | 12624 | 1.06 | 0.000723 |
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Target: 5'- cAUGAACGACCCAGAGCAGCUCGCGCUg -3' miRNA: 3'- -UACUUGCUGGGUCUCGUCGAGCGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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