Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18620 | 5' | -58.7 | NC_004682.1 | + | 12264 | 0.66 | 0.524682 |
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Target: 5'- cCGAGCaggUCGGCgcgauccgugaGGGCCGcauccuGGGuCGUCCc -3' miRNA: 3'- -GCUUG---AGCCGa----------CCUGGCu-----CCC-GCAGG- -5' |
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| 18620 | 5' | -58.7 | NC_004682.1 | + | 19343 | 0.66 | 0.545345 |
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Target: 5'- gGGACUCGGaaUGGcgcaaGgCGuGGGCGUUCg -3' miRNA: 3'- gCUUGAGCCg-ACC-----UgGCuCCCGCAGG- -5' |
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| 18620 | 5' | -58.7 | NC_004682.1 | + | 38190 | 0.66 | 0.555776 |
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Target: 5'- aCGGAgUCGacaGaaGGACCGGGGGC-UCUa -3' miRNA: 3'- -GCUUgAGC---CgaCCUGGCUCCCGcAGG- -5' |
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| 18620 | 5' | -58.7 | NC_004682.1 | + | 38055 | 0.66 | 0.566264 |
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Target: 5'- gGAucGCUUGGggGaGGCCGguuucGGGGCGUCa -3' miRNA: 3'- gCU--UGAGCCgaC-CUGGC-----UCCCGCAGg -5' |
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| 18620 | 5' | -58.7 | NC_004682.1 | + | 29850 | 0.66 | 0.566264 |
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Target: 5'- uCGAGCg-GGUUGu-CCGGGaGCGUCCa -3' miRNA: 3'- -GCUUGagCCGACcuGGCUCcCGCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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