miRNA: 3'- -GCUUgAGC---CgaCCUGGCUCCCGcAGG- -5'
miRNA: 3'- gCUUGAgCCGACCuGGCUCCcGCaGG- -5'
miRNA: 3'- -GCUUG-AGC-CGaCCUGgCucCCGCAGG- -5'
miRNA: 3'- gCUugAGcCGa-CCUGGc--UCCCGCAGg -5'
miRNA: 3'- gCUUGaGcCGACCUGGCUcCCgCAGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18620 | 5' | -58.7 | NC_004682.1 | + | 38190 | 0.66 | 0.555776 |
|
Target: 5'- aCGGAgUCGacaGaaGGACCGGGGGC-UCUa -3' miRNA: 3'- -GCUUgAGC---CgaCCUGGCUCCCGcAGG- -5' |
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| 18620 | 5' | -58.7 | NC_004682.1 | + | 42463 | 0.68 | 0.445492 |
|
Target: 5'- uGAACU-GGCUGG-CCGGGGuCGgCCu -3' miRNA: 3'- gCUUGAgCCGACCuGGCUCCcGCaGG- -5' |
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| 18620 | 5' | -58.7 | NC_004682.1 | + | 42530 | 0.66 | 0.514462 |
|
Target: 5'- gCGAACaUCGaGC-GGACgGu-GGCGUCCa -3' miRNA: 3'- -GCUUG-AGC-CGaCCUGgCucCCGCAGG- -5' |
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| 18620 | 5' | -58.7 | NC_004682.1 | + | 42971 | 0.67 | 0.455037 |
|
Target: 5'- gGGugUCaGCggGGGCCuccAGGGCGUCg -3' miRNA: 3'- gCUugAGcCGa-CCUGGc--UCCCGCAGg -5' |
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| 18620 | 5' | -58.7 | NC_004682.1 | + | 48614 | 0.67 | 0.484313 |
|
Target: 5'- gGGAC-CuGCUGGGCCGGuGG-GUCCg -3' miRNA: 3'- gCUUGaGcCGACCUGGCUcCCgCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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