Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18629 | 3' | -50.1 | NC_004682.1 | + | 14409 | 0.66 | 0.952548 |
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Target: 5'- -uCUUCGAUGA-GUUcgccgagaccaaGUcCGAGGAGGa -3' miRNA: 3'- ccGAAGCUACUaCAG------------CAuGCUCCUCC- -5' |
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| 18629 | 3' | -50.1 | NC_004682.1 | + | 49667 | 0.66 | 0.937983 |
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Target: 5'- cGGCggCGGUcacccUCGgagACGAGGAGGc -3' miRNA: 3'- -CCGaaGCUAcuac-AGCa--UGCUCCUCC- -5' |
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| 18629 | 3' | -50.1 | NC_004682.1 | + | 49495 | 0.67 | 0.914535 |
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Target: 5'- uGGCUuccuuucgcgUCGGUGAUGUacaGCcccgGAGGGGGa -3' miRNA: 3'- -CCGA----------AGCUACUACAgcaUG----CUCCUCC- -5' |
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| 18629 | 3' | -50.1 | NC_004682.1 | + | 29259 | 0.67 | 0.90795 |
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Target: 5'- cGGCggCGAUGAaGUCGgcauCGGccucgcucuGGAGGu -3' miRNA: 3'- -CCGaaGCUACUaCAGCau--GCU---------CCUCC- -5' |
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| 18629 | 3' | -50.1 | NC_004682.1 | + | 5108 | 0.68 | 0.896823 |
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Target: 5'- cGGCUUCagccacaccuggcagGA-GAUcuucGUCG-GCGAGGAGGg -3' miRNA: 3'- -CCGAAG---------------CUaCUA----CAGCaUGCUCCUCC- -5' |
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| 18629 | 3' | -50.1 | NC_004682.1 | + | 38044 | 0.68 | 0.886498 |
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Target: 5'- cGGCgUCGggGGgaUCGcUugGGGGAGGc -3' miRNA: 3'- -CCGaAGCuaCUacAGC-AugCUCCUCC- -5' |
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| 18629 | 3' | -50.1 | NC_004682.1 | + | 10018 | 0.69 | 0.840044 |
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Target: 5'- gGGCUacUCGAUcaccgaacgcgaagaGAUG-CGgaagugggACGAGGAGGa -3' miRNA: 3'- -CCGA--AGCUA---------------CUACaGCa-------UGCUCCUCC- -5' |
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| 18629 | 3' | -50.1 | NC_004682.1 | + | 31544 | 0.72 | 0.703492 |
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Target: 5'- cGC-UCGAUGAccuggagGUCGUACGAGGcguuguGGa -3' miRNA: 3'- cCGaAGCUACUa------CAGCAUGCUCCu-----CC- -5' |
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| 18629 | 3' | -50.1 | NC_004682.1 | + | 9243 | 1.14 | 0.00195 |
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Target: 5'- aGGCUUCGAUGAUGUCGUACGAGGAGGu -3' miRNA: 3'- -CCGAAGCUACUACAGCAUGCUCCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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