Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18631 | 5' | -62.7 | NC_004682.1 | + | 7932 | 0.66 | 0.36645 |
Target: 5'- -cGGGGgGCCGgCGGGCCAUGagcagUGGc -3' miRNA: 3'- guCUCCgCGGCgGCUCGGUGCa----GCCc -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 3056 | 0.66 | 0.36645 |
Target: 5'- -cGAGGCgGCUGUCGAGuUCugGgaaGGGa -3' miRNA: 3'- guCUCCG-CGGCGGCUC-GGugCag-CCC- -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 48832 | 0.66 | 0.358302 |
Target: 5'- -cGGGuGCGCCGUCG-GCC---UCGGGg -3' miRNA: 3'- guCUC-CGCGGCGGCuCGGugcAGCCC- -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 41456 | 0.66 | 0.358302 |
Target: 5'- --cAGGCGCuCGUCGAccgcGCCAUGUCGu- -3' miRNA: 3'- gucUCCGCG-GCGGCU----CGGUGCAGCcc -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 41454 | 0.66 | 0.350282 |
Target: 5'- gUAGAGGU-CCgacaGCCGAGUCcUGUUGGGg -3' miRNA: 3'- -GUCUCCGcGG----CGGCUCGGuGCAGCCC- -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 36317 | 0.66 | 0.350282 |
Target: 5'- aCGGAcuGGCGagUGCCaGGGCCGuCGUCGcGGg -3' miRNA: 3'- -GUCU--CCGCg-GCGG-CUCGGU-GCAGC-CC- -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 24916 | 0.66 | 0.342391 |
Target: 5'- uGGAGGCGCaaCCGcGCCguGCGUCuGGc -3' miRNA: 3'- gUCUCCGCGgcGGCuCGG--UGCAGcCC- -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 29386 | 0.66 | 0.334628 |
Target: 5'- cCAGGaaccacGGCGUguaguacuCGCCGGGgaACGUCGGGu -3' miRNA: 3'- -GUCU------CCGCG--------GCGGCUCggUGCAGCCC- -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 4553 | 0.66 | 0.326996 |
Target: 5'- cCAGAGGgaugGCCGCaacGCCGCGuucgcUCGGGc -3' miRNA: 3'- -GUCUCCg---CGGCGgcuCGGUGC-----AGCCC- -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 44697 | 0.66 | 0.324731 |
Target: 5'- gCAGcGGCccGCCGUCGuacgccuuggcguaGGCCGCGUagCGGGc -3' miRNA: 3'- -GUCuCCG--CGGCGGC--------------UCGGUGCA--GCCC- -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 26228 | 0.67 | 0.319492 |
Target: 5'- -cGuGGCGuUCGCCGGGUCGgaGUCGGa -3' miRNA: 3'- guCuCCGC-GGCGGCUCGGUg-CAGCCc -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 36547 | 0.67 | 0.297762 |
Target: 5'- gGGAGGCuuggacucGCCGCCcugcggcuuGCCGcCGUCGGa -3' miRNA: 3'- gUCUCCG--------CGGCGGcu-------CGGU-GCAGCCc -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 23671 | 0.67 | 0.293556 |
Target: 5'- aAGuGG-GUCGCCGAGCagggccacacgguCGUCGGGu -3' miRNA: 3'- gUCuCCgCGGCGGCUCGgu-----------GCAGCCC- -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 16653 | 0.67 | 0.290778 |
Target: 5'- cCAGcAGGCGCUGCgCG-GCaUGCGcUCGGGc -3' miRNA: 3'- -GUC-UCCGCGGCG-GCuCG-GUGC-AGCCC- -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 25840 | 0.67 | 0.290778 |
Target: 5'- gCAGA--UGCCGCCGAGUCGgCGUCGu- -3' miRNA: 3'- -GUCUccGCGGCGGCUCGGU-GCAGCcc -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 11489 | 0.67 | 0.283244 |
Target: 5'- aGGAGGCcggugcuacggcuGCCGCCGcGCCaACGgcaagCGGa -3' miRNA: 3'- gUCUCCG-------------CGGCGGCuCGG-UGCa----GCCc -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 45937 | 0.68 | 0.257779 |
Target: 5'- -cGuGGUGCCGcCCGAGUaGCG-CGGGc -3' miRNA: 3'- guCuCCGCGGC-GGCUCGgUGCaGCCC- -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 4719 | 0.68 | 0.257779 |
Target: 5'- ---cGGCGCUGCCGAcgcgGCCACGaagUGGa -3' miRNA: 3'- gucuCCGCGGCGGCU----CGGUGCa--GCCc -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 35375 | 0.68 | 0.245462 |
Target: 5'- cCAG-GGCaGCCGCCcAGCCcuCG-CGGGa -3' miRNA: 3'- -GUCuCCG-CGGCGGcUCGGu-GCaGCCC- -5' |
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18631 | 5' | -62.7 | NC_004682.1 | + | 48168 | 0.69 | 0.239488 |
Target: 5'- gCAGAGGuCGCUGCC--GCCACacCGGGc -3' miRNA: 3'- -GUCUCC-GCGGCGGcuCGGUGcaGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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