Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18632 | 5' | -54.4 | NC_004682.1 | + | 10167 | 0.66 | 0.75048 |
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Target: 5'- aCCCgGCuGA--GGaGGCUGCUGCGUGa -3' miRNA: 3'- -GGGgCGuCUagCCcCUGAUGAUGUAC- -5' |
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| 18632 | 5' | -54.4 | NC_004682.1 | + | 6053 | 0.66 | 0.75048 |
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Target: 5'- cCCCUGCGGGUCGGGucgaacGACgccaaUACcgGc -3' miRNA: 3'- -GGGGCGUCUAGCCC------CUGaug--AUGuaC- -5' |
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| 18632 | 5' | -54.4 | NC_004682.1 | + | 21076 | 0.67 | 0.697118 |
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Target: 5'- uUCCCGCuccUCGGGGACaACUcCAa- -3' miRNA: 3'- -GGGGCGucuAGCCCCUGaUGAuGUac -5' |
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| 18632 | 5' | -54.4 | NC_004682.1 | + | 20765 | 0.68 | 0.664244 |
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Target: 5'- aCCCCG-AGGUCGGaGGACggu--CGUGg -3' miRNA: 3'- -GGGGCgUCUAGCC-CCUGaugauGUAC- -5' |
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| 18632 | 5' | -54.4 | NC_004682.1 | + | 7083 | 0.7 | 0.511467 |
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Target: 5'- cCCCCGCAuguGAUCGGGc----CUACGUGg -3' miRNA: 3'- -GGGGCGU---CUAGCCCcugauGAUGUAC- -5' |
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| 18632 | 5' | -54.4 | NC_004682.1 | + | 12474 | 0.77 | 0.198842 |
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Target: 5'- uCCUCGCGGA-CGGGGACgagccgggGCUGCAg- -3' miRNA: 3'- -GGGGCGUCUaGCCCCUGa-------UGAUGUac -5' |
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| 18632 | 5' | -54.4 | NC_004682.1 | + | 7420 | 1.11 | 0.00085 |
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Target: 5'- cCCCCGCAGAUCGGGGACUACUACAUGg -3' miRNA: 3'- -GGGGCGUCUAGCCCCUGAUGAUGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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