Results 1 - 20 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18639 | 5' | -56.4 | NC_004682.1 | + | 43556 | 0.66 | 0.627794 |
Target: 5'- uGCCcucgcgguagacguaGUUGCCGuuGUCAUCGaCCGCGu -3' miRNA: 3'- uCGG---------------UAGCGGCuuCGGUAGUaGGCGU- -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 6418 | 0.66 | 0.623357 |
Target: 5'- cGCUugGUCuGcCCGAucCCGUCGUCCGCGu -3' miRNA: 3'- uCGG--UAG-C-GGCUucGGUAGUAGGCGU- -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 48838 | 0.66 | 0.623357 |
Target: 5'- cGCCGUCgGCCucggGggGCCAaCcgCCGUc -3' miRNA: 3'- uCGGUAG-CGG----CuuCGGUaGuaGGCGu -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 13838 | 0.66 | 0.623357 |
Target: 5'- aGGCCGgaagGCCGAGGCCAaccuggcaCAggcCCGCGc -3' miRNA: 3'- -UCGGUag--CGGCUUCGGUa-------GUa--GGCGU- -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 32316 | 0.66 | 0.612271 |
Target: 5'- uGUCcgCGagCGAGGCCAUCuggcUCUGCAu -3' miRNA: 3'- uCGGuaGCg-GCUUCGGUAGu---AGGCGU- -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 19936 | 0.66 | 0.612271 |
Target: 5'- -cCCGcUCGCCGAGGCgAUCA-CCGa- -3' miRNA: 3'- ucGGU-AGCGGCUUCGgUAGUaGGCgu -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 22252 | 0.66 | 0.612271 |
Target: 5'- aGGCCGgaUCGCucCGguGCCAUCuggGUCCGUu -3' miRNA: 3'- -UCGGU--AGCG--GCuuCGGUAG---UAGGCGu -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 34313 | 0.66 | 0.612271 |
Target: 5'- uAGCCcugcCGcCCGuagucGGCCAgCAUCCGCGu -3' miRNA: 3'- -UCGGua--GC-GGCu----UCGGUaGUAGGCGU- -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 4954 | 0.66 | 0.611163 |
Target: 5'- cGGCUAcUCGCgcugggaCGAAGCCAUCA-CCGa- -3' miRNA: 3'- -UCGGU-AGCG-------GCUUCGGUAGUaGGCgu -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 30935 | 0.66 | 0.605626 |
Target: 5'- cGCCGucggaUCGCCGAcgaGGCUgAUCuguuggaccucgcacGUCCGCAg -3' miRNA: 3'- uCGGU-----AGCGGCU---UCGG-UAG---------------UAGGCGU- -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 48947 | 0.66 | 0.601202 |
Target: 5'- uGGCCGUCGaugUGGAGuCCcUCGcCCGCAg -3' miRNA: 3'- -UCGGUAGCg--GCUUC-GGuAGUaGGCGU- -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 42318 | 0.66 | 0.59016 |
Target: 5'- uGCCGUCGUgGAAGaCCucuugcucgAUCA-CCGCGu -3' miRNA: 3'- uCGGUAGCGgCUUC-GG---------UAGUaGGCGU- -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 47405 | 0.66 | 0.579154 |
Target: 5'- uGUCcggAUCGCCGggGCCGUCGaaccucagucccUUCGUc -3' miRNA: 3'- uCGG---UAGCGGCuuCGGUAGU------------AGGCGu -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 30679 | 0.67 | 0.568193 |
Target: 5'- gGGCCugguaGUCGaCUGAGGCCAUCAaUUgGCc -3' miRNA: 3'- -UCGG-----UAGC-GGCUUCGGUAGU-AGgCGu -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 31761 | 0.67 | 0.568193 |
Target: 5'- gAGCCcggacgaaGUCGCgGAAGCCccagagGUCAUCCuCGu -3' miRNA: 3'- -UCGG--------UAGCGgCUUCGG------UAGUAGGcGU- -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 4754 | 0.67 | 0.546436 |
Target: 5'- -aCCA-CGCCGAAGCCGaCGaCUGCGg -3' miRNA: 3'- ucGGUaGCGGCUUCGGUaGUaGGCGU- -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 33711 | 0.67 | 0.524953 |
Target: 5'- aGGaCCcgCGCCgGAAGCCAgugCGUCgGUg -3' miRNA: 3'- -UC-GGuaGCGG-CUUCGGUa--GUAGgCGu -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 44667 | 0.67 | 0.514331 |
Target: 5'- cGUCGUCguaGCCGAaauAGCCGUCGaCuCGCAg -3' miRNA: 3'- uCGGUAG---CGGCU---UCGGUAGUaG-GCGU- -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 10147 | 0.68 | 0.503797 |
Target: 5'- cGGCaagcgCGCCGggGUCA---UCCGCAa -3' miRNA: 3'- -UCGgua--GCGGCuuCGGUaguAGGCGU- -5' |
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18639 | 5' | -56.4 | NC_004682.1 | + | 4370 | 0.68 | 0.483021 |
Target: 5'- cGCCGUCaCCGAAGCCg----CCGCc -3' miRNA: 3'- uCGGUAGcGGCUUCGGuaguaGGCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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