Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18645 | 3' | -61.4 | NC_004682.1 | + | 40160 | 0.66 | 0.373762 |
Target: 5'- gGCCCCgaGGUACaCAG-GCCCUCGGg- -3' miRNA: 3'- -UGGGGg-UCAUGgGUCaUGGGGGCCau -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 37491 | 0.66 | 0.348757 |
Target: 5'- gGCCCCCcg-AUCCAcGUugCCCCaGUGg -3' miRNA: 3'- -UGGGGGucaUGGGU-CAugGGGGcCAU- -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 44575 | 0.66 | 0.348757 |
Target: 5'- cGCCUCCAG-GCCCAGaGCCCggaGGUc -3' miRNA: 3'- -UGGGGGUCaUGGGUCaUGGGgg-CCAu -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 133 | 0.67 | 0.317312 |
Target: 5'- gGCaCCCuGUGCCCAGUGCCCUg---- -3' miRNA: 3'- -UGgGGGuCAUGGGUCAUGGGGgccau -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 232 | 0.67 | 0.307562 |
Target: 5'- -aCCCCAGgcgggguguaccccUACCCGGUACCCCa---- -3' miRNA: 3'- ugGGGGUC--------------AUGGGUCAUGGGGgccau -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 329 | 0.67 | 0.302408 |
Target: 5'- cACCCCC--UGCCCuGUaggcACCCCCuGUAg -3' miRNA: 3'- -UGGGGGucAUGGGuCA----UGGGGGcCAU- -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 13447 | 0.67 | 0.29516 |
Target: 5'- gGCCUCgGGaguaccucACCCGGUACgCCCCGGc- -3' miRNA: 3'- -UGGGGgUCa-------UGGGUCAUG-GGGGCCau -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 31672 | 0.68 | 0.26095 |
Target: 5'- gACCCUucacauCAGUACCgAGguguacCCCCCGGUc -3' miRNA: 3'- -UGGGG------GUCAUGGgUCau----GGGGGCCAu -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 17416 | 0.69 | 0.224241 |
Target: 5'- cGCgCCCCAGgcgGCUCAGgagGCCCCCa--- -3' miRNA: 3'- -UG-GGGGUCa--UGGGUCa--UGGGGGccau -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 35 | 0.72 | 0.135855 |
Target: 5'- uACCCCCcuggGGUACCUGGcaUACCCCCGa-- -3' miRNA: 3'- -UGGGGG----UCAUGGGUC--AUGGGGGCcau -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 30415 | 0.73 | 0.115389 |
Target: 5'- aGCCCCCAGgGCCUAGUccauGCCCgUGGg- -3' miRNA: 3'- -UGGGGGUCaUGGGUCA----UGGGgGCCau -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 285 | 1.06 | 0.000373 |
Target: 5'- uACCCCCAGUACCCAGUACCCCCGGUAg -3' miRNA: 3'- -UGGGGGUCAUGGGUCAUGGGGGCCAU- -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 37036 | 0.68 | 0.267524 |
Target: 5'- uGCUgCCAGUugCCAGUuccACCCUugaGGUAg -3' miRNA: 3'- -UGGgGGUCAugGGUCA---UGGGGg--CCAU- -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 44735 | 0.67 | 0.302408 |
Target: 5'- cGCCgCCGGUGgCCAcGUACUCCCcGUGg -3' miRNA: 3'- -UGGgGGUCAUgGGU-CAUGGGGGcCAU- -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 12617 | 0.67 | 0.320358 |
Target: 5'- uCCCuugacagagaacgggCCGGggGCCUAGaGCCCCCGGUc -3' miRNA: 3'- uGGG---------------GGUCa-UGGGUCaUGGGGGCCAu -5' |
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18645 | 3' | -61.4 | NC_004682.1 | + | 7736 | 0.66 | 0.365294 |
Target: 5'- gAUCCCCGGUGCCC-GU-CgCCUGGc- -3' miRNA: 3'- -UGGGGGUCAUGGGuCAuGgGGGCCau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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