miRNA: 3'- -GUGuuACAGa-ACC-GGCACGuGCGUCc -5'
miRNA: 3'- gUguUACAGaaCCgGCACGugCGUCC- -5'
miRNA: 3'- gUguUACAGaaCCgGCACGugCGUCC- -5'
miRNA: 3'- -GUGUUACAG----AACCG--GCACGUgCGUCC- -5'
miRNA: 3'- gUGUUA-CAGaACCGGCACGUGCg----UCC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1865 | 5' | -53.1 | NC_001347.2 | + | 61559 | 0.71 | 0.892546 |
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Target: 5'- cCGCuuUGUCcgUGGcCCGUGCcCGCAGc -3' miRNA: 3'- -GUGuuACAGa-ACC-GGCACGuGCGUCc -5' |
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| 1865 | 5' | -53.1 | NC_001347.2 | + | 709 | 0.72 | 0.85667 |
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Target: 5'- gGguGUGUCggGGgUGUGUugGCAGGg -3' miRNA: 3'- gUguUACAGaaCCgGCACGugCGUCC- -5' |
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| 1865 | 5' | -53.1 | NC_001347.2 | + | 230188 | 0.72 | 0.85667 |
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Target: 5'- gGguGUGUCggGGgUGUGUugGCAGGg -3' miRNA: 3'- gUguUACAGaaCCgGCACGugCGUCC- -5' |
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| 1865 | 5' | -53.1 | NC_001347.2 | + | 77446 | 0.75 | 0.682926 |
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Target: 5'- gCACGAUGUCguggUUGGCggCGUGCA-GCAGGu -3' miRNA: 3'- -GUGUUACAG----AACCG--GCACGUgCGUCC- -5' |
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| 1865 | 5' | -53.1 | NC_001347.2 | + | 155579 | 0.76 | 0.642396 |
|
Target: 5'- gGCGAUgGUCaUGGCCGUGCGCGaagugAGGc -3' miRNA: 3'- gUGUUA-CAGaACCGGCACGUGCg----UCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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