miRNA: 3'- gUGUUACAGaaCCGGCaCgUGCgUCC- -5'
miRNA: 3'- -GUGU---UACagaACCGGCaCGUGCGuCC- -5'
miRNA: 3'- gUGuUACagaACCGGCACGugC-GuCC- -5'
miRNA: 3'- gUguUACAGaaCCgGCACGugCGUCC- -5'
miRNA: 3'- gUGUUACAGAACCgGCACG----UGCGUCc -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1865 | 5' | -53.1 | NC_001347.2 | + | 189630 | 0.69 | 0.938153 |
|
Target: 5'- gACGGUGUUggGGCCGgGgACGgGGGu -3' miRNA: 3'- gUGUUACAGaaCCGGCaCgUGCgUCC- -5' |
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| 1865 | 5' | -53.1 | NC_001347.2 | + | 203430 | 0.66 | 0.98973 |
|
Target: 5'- uCACGcccGUGgugUGGUCG-GCACGCuGGg -3' miRNA: 3'- -GUGU---UACagaACCGGCaCGUGCGuCC- -5' |
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| 1865 | 5' | -53.1 | NC_001347.2 | + | 205945 | 0.68 | 0.968954 |
|
Target: 5'- gGCcGUGg--UGGCCGUGCugGgCuGGg -3' miRNA: 3'- gUGuUACagaACCGGCACGugC-GuCC- -5' |
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| 1865 | 5' | -53.1 | NC_001347.2 | + | 230188 | 0.72 | 0.85667 |
|
Target: 5'- gGguGUGUCggGGgUGUGUugGCAGGg -3' miRNA: 3'- gUguUACAGaaCCgGCACGugCGUCC- -5' |
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| 1865 | 5' | -53.1 | NC_001347.2 | + | 230256 | 0.7 | 0.917225 |
|
Target: 5'- gGCGGUGUUUUGGgUGUGUcgggGCGCGGc -3' miRNA: 3'- gUGUUACAGAACCgGCACG----UGCGUCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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