Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18650 | 3' | -52.2 | NC_004682.1 | + | 33206 | 0.7 | 0.612649 |
Target: 5'- cCGGCUACaccgacucggggaCACCAUCGUCGGCUgaCGAa -3' miRNA: 3'- aGUCGAUGa------------GUGGUGGCAGUUGA--GCU- -5' |
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18650 | 3' | -52.2 | NC_004682.1 | + | 3772 | 0.74 | 0.424338 |
Target: 5'- gUCAGCgacgACUC-CCGCCGuggcggUCAGCUCGc -3' miRNA: 3'- -AGUCGa---UGAGuGGUGGC------AGUUGAGCu -5' |
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18650 | 3' | -52.2 | NC_004682.1 | + | 14809 | 0.74 | 0.423362 |
Target: 5'- cUCGGUg--UCACCACCGUCGaagccgaACUCGGg -3' miRNA: 3'- -AGUCGaugAGUGGUGGCAGU-------UGAGCU- -5' |
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18650 | 3' | -52.2 | NC_004682.1 | + | 7266 | 0.75 | 0.368245 |
Target: 5'- cCAGCgUGCUCACCGCCGgggguCUUGAc -3' miRNA: 3'- aGUCG-AUGAGUGGUGGCaguu-GAGCU- -5' |
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18650 | 3' | -52.2 | NC_004682.1 | + | 5207 | 0.75 | 0.34217 |
Target: 5'- -aGGCcgACUgCACCACCGUCAAC-CGGa -3' miRNA: 3'- agUCGa-UGA-GUGGUGGCAGUUGaGCU- -5' |
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18650 | 3' | -52.2 | NC_004682.1 | + | 1131 | 1.1 | 0.001658 |
Target: 5'- gUCAGCUACUCACCACCGUCAACUCGAu -3' miRNA: 3'- -AGUCGAUGAGUGGUGGCAGUUGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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