Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18650 | 5' | -60.2 | NC_004682.1 | + | 32074 | 0.66 | 0.486372 |
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Target: 5'- aUCCUCGUCUcgccgcCCGAGcagccGGUccagauGGCCGUCGc -3' miRNA: 3'- gAGGAGCAGA------GGCUC-----CCA------CUGGCGGC- -5' |
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| 18650 | 5' | -60.2 | NC_004682.1 | + | 28201 | 0.66 | 0.47657 |
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Target: 5'- gUCCUCGgCUUCGAGGucucUGuccgcgcucGCCGCCGc -3' miRNA: 3'- gAGGAGCaGAGGCUCCc---AC---------UGGCGGC- -5' |
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| 18650 | 5' | -60.2 | NC_004682.1 | + | 25896 | 0.67 | 0.41997 |
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Target: 5'- uCUUgUCgGUCUCCu-GGGUGACCcaGCCa -3' miRNA: 3'- -GAGgAG-CAGAGGcuCCCACUGG--CGGc -5' |
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| 18650 | 5' | -60.2 | NC_004682.1 | + | 43496 | 0.67 | 0.41997 |
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Target: 5'- ---gUCGUCaCCGGGGGUG-UUGCCGg -3' miRNA: 3'- gaggAGCAGaGGCUCCCACuGGCGGC- -5' |
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| 18650 | 5' | -60.2 | NC_004682.1 | + | 26868 | 0.67 | 0.402025 |
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Target: 5'- -aCCgCGUgCUgCGGGGGaugGACCGCUGg -3' miRNA: 3'- gaGGaGCA-GAgGCUCCCa--CUGGCGGC- -5' |
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| 18650 | 5' | -60.2 | NC_004682.1 | + | 27902 | 0.67 | 0.402025 |
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Target: 5'- gUCCUuuucgCGUUUCUGGGGGUuuCCGaCCGg -3' miRNA: 3'- gAGGA-----GCAGAGGCUCCCAcuGGC-GGC- -5' |
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| 18650 | 5' | -60.2 | NC_004682.1 | + | 13896 | 0.68 | 0.366799 |
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Target: 5'- aUCUUCGggccggaucaccugaCGAGGGUGACCGUCa -3' miRNA: 3'- gAGGAGCagag-----------GCUCCCACUGGCGGc -5' |
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| 18650 | 5' | -60.2 | NC_004682.1 | + | 44574 | 0.7 | 0.277065 |
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Target: 5'- uUCC-CGcUCUUCGGGGGUGGCCucaacguaucGCCa -3' miRNA: 3'- gAGGaGC-AGAGGCUCCCACUGG----------CGGc -5' |
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| 18650 | 5' | -60.2 | NC_004682.1 | + | 1168 | 1.07 | 0.000471 |
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Target: 5'- cCUCCUCGUCUCCGAGGGUGACCGCCGc -3' miRNA: 3'- -GAGGAGCAGAGGCUCCCACUGGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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