Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18666 | 5' | -56 | NC_004682.1 | + | 13767 | 0.66 | 0.622521 |
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Target: 5'- gCGGGCGGUGCgc--CGAGaGCugUUGu -3' miRNA: 3'- -GCCCGCCACGagcuGUUCaUGugGAC- -5' |
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| 18666 | 5' | -56 | NC_004682.1 | + | 35862 | 0.67 | 0.611523 |
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Target: 5'- uGcGGCGcGUGgUCGACGGGa--ACCUGg -3' miRNA: 3'- gC-CCGC-CACgAGCUGUUCaugUGGAC- -5' |
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| 18666 | 5' | -56 | NC_004682.1 | + | 14159 | 0.67 | 0.600541 |
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Target: 5'- cCGGGCGGcGCUgccugaCGugAAGguggGCuCCUGg -3' miRNA: 3'- -GCCCGCCaCGA------GCugUUCa---UGuGGAC- -5' |
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| 18666 | 5' | -56 | NC_004682.1 | + | 5429 | 0.68 | 0.535517 |
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Target: 5'- cCGGGCGuaGUGCUCGcgcCAGGUG-AUCUGa -3' miRNA: 3'- -GCCCGC--CACGAGCu--GUUCAUgUGGAC- -5' |
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| 18666 | 5' | -56 | NC_004682.1 | + | 41771 | 0.68 | 0.524897 |
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Target: 5'- aGGGCGGUGCgugagggCGACcuGGgggACGCUg- -3' miRNA: 3'- gCCCGCCACGa------GCUGu-UCa--UGUGGac -5' |
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| 18666 | 5' | -56 | NC_004682.1 | + | 4828 | 0.7 | 0.424061 |
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Target: 5'- uGGGCGGaucGUUCGuCAGGUAgACCg- -3' miRNA: 3'- gCCCGCCa--CGAGCuGUUCAUgUGGac -5' |
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| 18666 | 5' | -56 | NC_004682.1 | + | 6367 | 1.09 | 0.000763 |
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Target: 5'- gCGGGCGGUGCUCGACAAGUACACCUGg -3' miRNA: 3'- -GCCCGCCACGAGCUGUUCAUGUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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