Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18667 | 5' | -56.8 | NC_004682.1 | + | 50896 | 0.66 | 0.569731 |
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Target: 5'- aGGGGCgGGGGAGGGCAUaccccccUGGGGu -3' miRNA: 3'- -UCCCGaCCUCUUCCGUGgcaa---GCUCU- -5' |
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| 18667 | 5' | -56.8 | NC_004682.1 | + | 37519 | 0.67 | 0.548142 |
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Target: 5'- cGGGC-GGucuacGAAGGC-CCGUUCGGc- -3' miRNA: 3'- uCCCGaCCu----CUUCCGuGGCAAGCUcu -5' |
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| 18667 | 5' | -56.8 | NC_004682.1 | + | 15271 | 0.67 | 0.548142 |
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Target: 5'- gGGGGgaGGGGAAGGC-CCGccaaGGAa -3' miRNA: 3'- -UCCCgaCCUCUUCCGuGGCaagcUCU- -5' |
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| 18667 | 5' | -56.8 | NC_004682.1 | + | 43133 | 0.67 | 0.526815 |
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Target: 5'- cAGGaGCUGcGuaAGGAGGCACCGUgaCGGuGAa -3' miRNA: 3'- -UCC-CGAC-C--UCUUCCGUGGCAa-GCU-CU- -5' |
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| 18667 | 5' | -56.8 | NC_004682.1 | + | 48455 | 0.68 | 0.464938 |
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Target: 5'- gGGGGCgaugcuccUGGGcgaccagaucGAAGGCACCGaUUCGAc- -3' miRNA: 3'- -UCCCG--------ACCU----------CUUCCGUGGC-AAGCUcu -5' |
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| 18667 | 5' | -56.8 | NC_004682.1 | + | 4477 | 0.68 | 0.454 |
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Target: 5'- gGGGGCUGGAGAucuuccgGGGC-CUGacgCGAuGAc -3' miRNA: 3'- -UCCCGACCUCU-------UCCGuGGCaa-GCU-CU- -5' |
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| 18667 | 5' | -56.8 | NC_004682.1 | + | 7323 | 0.74 | 0.20794 |
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Target: 5'- aAGGGCUGGGGcAAGGaC-CCGUUCGcAGc -3' miRNA: 3'- -UCCCGACCUC-UUCC-GuGGCAAGC-UCu -5' |
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| 18667 | 5' | -56.8 | NC_004682.1 | + | 6717 | 1.08 | 0.000664 |
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Target: 5'- aAGGGCUGGAGAAGGCACCGUUCGAGAu -3' miRNA: 3'- -UCCCGACCUCUUCCGUGGCAAGCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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