Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18668 | 5' | -51.1 | NC_004682.1 | + | 4299 | 0.66 | 0.908995 |
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Target: 5'- cCGAUCAUCGcgaaGCAgauCGCUGaCGaGUUCggCa -3' miRNA: 3'- -GCUAGUAGC----UGU---GCGAC-GC-CAAGaaG- -5' |
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| 18668 | 5' | -51.1 | NC_004682.1 | + | 32903 | 0.67 | 0.854928 |
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Target: 5'- gGAUCugAUCGACGCGCcgggacGCGGcuaCUUCc -3' miRNA: 3'- gCUAG--UAGCUGUGCGa-----CGCCaa-GAAG- -5' |
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| 18668 | 5' | -51.1 | NC_004682.1 | + | 13035 | 0.67 | 0.835298 |
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Target: 5'- uGAUCAuccgcUCGACaucagccaaacggACGUUgaucagcGCGGUUCUUCg -3' miRNA: 3'- gCUAGU-----AGCUG-------------UGCGA-------CGCCAAGAAG- -5' |
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| 18668 | 5' | -51.1 | NC_004682.1 | + | 18806 | 0.68 | 0.808734 |
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Target: 5'- aGuUCGUCGAUGCGCUGCuggaGGgcugcCUUCg -3' miRNA: 3'- gCuAGUAGCUGUGCGACG----CCaa---GAAG- -5' |
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| 18668 | 5' | -51.1 | NC_004682.1 | + | 13871 | 0.7 | 0.691902 |
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Target: 5'- cCGcgCAUCGACGCGCUGgCacaaGaUCUUCg -3' miRNA: 3'- -GCuaGUAGCUGUGCGAC-Gc---CaAGAAG- -5' |
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| 18668 | 5' | -51.1 | NC_004682.1 | + | 32848 | 0.7 | 0.680662 |
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Target: 5'- aGAUCcUUGAC-CGCUgGCGGUUCg-- -3' miRNA: 3'- gCUAGuAGCUGuGCGA-CGCCAAGaag -5' |
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| 18668 | 5' | -51.1 | NC_004682.1 | + | 44001 | 0.71 | 0.669372 |
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Target: 5'- uGAgcugaGACACGCUGCGGUgCUUa -3' miRNA: 3'- gCUaguagCUGUGCGACGCCAaGAAg -5' |
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| 18668 | 5' | -51.1 | NC_004682.1 | + | 6932 | 1.11 | 0.001785 |
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Target: 5'- gCGAUCAUCGACACGCUGCGGUUCUUCa -3' miRNA: 3'- -GCUAGUAGCUGUGCGACGCCAAGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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