miRNA: 3'- -CUCC-CgUugGACUGGU---------------UCUAG-GGGCC- -5'
miRNA: 3'- -CUcCCGUUg----GAC-UGGU--UCUAGGGGCC- -5'
miRNA: 3'- -CUCCCGuUGGACuGgUUCUaGGg-GCC- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18671 | 3' | -56.7 | NC_004682.1 | + | 40986 | 0.67 | 0.587595 |
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Target: 5'- uGAGGcGgGugCUGAUCAccggcagccgcgacuGGGUCgCCCGGa -3' miRNA: 3'- -CUCC-CgUugGACUGGU---------------UCUAG-GGGCC- -5' |
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| 18671 | 3' | -56.7 | NC_004682.1 | + | 41198 | 0.66 | 0.594123 |
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Target: 5'- cGAaGGCGAaccgaCUGcACCAgcAGAUCCUCGGc -3' miRNA: 3'- -CUcCCGUUg----GAC-UGGU--UCUAGGGGCC- -5' |
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| 18671 | 3' | -56.7 | NC_004682.1 | + | 13089 | 0.66 | 0.637842 |
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Target: 5'- cGAGGGCuACCUGuCgGAGA-CCgaCGGc -3' miRNA: 3'- -CUCCCGuUGGACuGgUUCUaGGg-GCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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