Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
18671 | 3' | -56.7 | NC_004682.1 | + | 12035 | 0.73 | 0.272579 |
Target: 5'- aAGGGCuACCUGGCCGAGAcgaCCaaGGu -3' miRNA: 3'- cUCCCGuUGGACUGGUUCUa--GGggCC- -5' |
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18671 | 3' | -56.7 | NC_004682.1 | + | 14920 | 0.76 | 0.156647 |
Target: 5'- cGAGcGGCGACCcGACCGAGAaggcgUUCCUGGu -3' miRNA: 3'- -CUC-CCGUUGGaCUGGUUCU-----AGGGGCC- -5' |
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18671 | 3' | -56.7 | NC_004682.1 | + | 7718 | 1.1 | 0.000579 |
Target: 5'- cGAGGGCAACCUGACCAAGAUCCCCGGu -3' miRNA: 3'- -CUCCCGUUGGACUGGUUCUAGGGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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