Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18678 | 3' | -56.4 | NC_004682.1 | + | 41100 | 0.66 | 0.635937 |
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Target: 5'- -gUCGCgaucaGGUCGCCGAggaucUGCuGgUGCa -3' miRNA: 3'- gaAGCGag---CCAGUGGCU-----GCGuCgACG- -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 43733 | 0.69 | 0.476092 |
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Target: 5'- -aUCGCUCG--CGCCGuCGUAGCgGCc -3' miRNA: 3'- gaAGCGAGCcaGUGGCuGCGUCGaCG- -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 45647 | 0.66 | 0.624983 |
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Target: 5'- --aUGCUCGccgCGCUGGgGCuGCUGCu -3' miRNA: 3'- gaaGCGAGCca-GUGGCUgCGuCGACG- -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 46120 | 0.67 | 0.602012 |
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Target: 5'- aCUUCGUggccgcgUCGGcagCGCCGAgCGaCAGCaGCa -3' miRNA: 3'- -GAAGCG-------AGCCa--GUGGCU-GC-GUCGaCG- -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 46201 | 0.76 | 0.183223 |
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Target: 5'- -gUCGCUCGGcgCuGCCGACGCGGCc-- -3' miRNA: 3'- gaAGCGAGCCa-G-UGGCUGCGUCGacg -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 46337 | 0.76 | 0.164449 |
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Target: 5'- gUUCGCUCGGgCGCUGGCG-AGCUGa -3' miRNA: 3'- gAAGCGAGCCaGUGGCUGCgUCGACg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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